Characterization of Plasmids That Encode for the K88 Colonization Antigen

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1978 May 1

PMID 352948

Citations 22

Authors

P L Shipley

C L Gyles

S Falkow

Affiliations

Soon will be listed here.

Abstract

K88 antigen, and important virulence factor in porcine enteropathogenic Escherichia coli (EEC), can be transferred along with the ability to ferment the trisaccharide raffinose (Raf). The plasmids from a number of EEC strains that encode these two properties were isolated and characterized. In most strains the K88 and Raf genes were found on a single nonconjugative plasmid approximately 50 x 10(6) daltons in size. This plasmid core was conserved with only slight variation among the strains tested. In some transconjugants, larger conjugative plasmids were observed that were apparently recombinants between the Raf/K88 plasmid and a transfer fa(tor. Occasionally plasmids carrying only the raffinose fermentation genes arose by deletion of a deoxyribonucleic acid segment of about 20 x 10(6) daltons that included the K88 antigen gene(s).

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References

Kornberg A, Zimmerman S, Kornberg S, Josse J . ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. INFLUENCE OF BACTERIOPHAGE T2 ON THE SYNTHETIC PATHWAY IN HOST CELLS. Proc Natl Acad Sci U S A. 1959; 45(6):772-85. PMC: 222636. DOI: 10.1073/pnas.45.6.772. View

Rutter J, Jones G, Brown G, Burrows M, Luther P . Antibacterial activity in colostrum and milk associated with protection of piglets against enteric disease caused by K88-positive Escherichia coli. Infect Immun. 1976; 13(3):667-76. PMC: 420662. DOI: 10.1128/iai.13.3.667-676.1976. View

ORSKOV I, ORSKOV F, SOJKA W, LEACH J . Simultaneous occurrence of E. coli B and Lantigens in strains from diseased swine. Influence of cultivation temperature. Two new E. coli Kantigens: K 87 and K 88. Acta Pathol Microbiol Scand. 1961; 53:404-22. View

Chilton M, Drummond M, Merio D, Sciaky D, Montoya A, Gordon M . Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis. Cell. 1977; 11(2):263-71. DOI: 10.1016/0092-8674(77)90043-5. View

Bak A, Christiansen G, Christiansen C, STENDERUP A, ORSKOV I, ORSKOV F . Circular DNA molecules controlling synthesis and transfer of the surface antigen (K88) in Escherichia coli. J Gen Microbiol. 1972; 73(2):373-85. DOI: 10.1099/00221287-73-2-373. View

Smith H, Parsell Z . Transmissible substrate-utilizing ability in enterobacteria. J Gen Microbiol. 1975; 87(1):129-40. DOI: 10.1099/00221287-87-1-129. View

Jones G, Rutter J . Contribution of the K88 antigen of Escherichia coli to enteropathogenicity; protection against disease by neutralizing the adhesive properties of K88 antigen. Am J Clin Nutr. 1974; 27(12):1441-9. DOI: 10.1093/ajcn/27.12.1441. View

Jones G, Rutter J . The association of K88 antigen with haemagglutinating activity in porcine strains of Escherichia coli. J Gen Microbiol. 1974; 84(1):135-44. DOI: 10.1099/00221287-84-1-135. View

Moon H . Pathogenesis of enteric diseases caused by Escherichia coli. Adv Vet Sci Comp Med. 1974; 18(0):179-211. View

10.

Jones G, Rutter J . Role of the K88 antigen in the pathogenesis of neonatal diarrhea caused by Escherichia coli in piglets. Infect Immun. 1972; 6(6):918-27. PMC: 419313. DOI: 10.1128/iai.6.6.918-927.1972. View

11.

Guerry P, Leblanc D, Falkow S . General method for the isolation of plasmid deoxyribonucleic acid. J Bacteriol. 1973; 116(2):1064-6. PMC: 285495. DOI: 10.1128/jb.116.2.1064-1066.1973. View

12.

Gyles C, So M, Falkow S . The enterotoxin plasmids of Escherichia coli. J Infect Dis. 1974; 130(1):40-9. DOI: 10.1093/infdis/130.1.40. View

13.

Anderson E, Natkin E . Transduction of resistance determinants and R factors of the transfer systems by phage Plkc. Mol Gen Genet. 1972; 114(3):261-5. DOI: 10.1007/BF01788895. View

14.

Cohen S, Chang A, Hsu L . Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A. 1972; 69(8):2110-4. PMC: 426879. DOI: 10.1073/pnas.69.8.2110. View

15.

Crosa J, Brenner D, Falkow S . Use of a single-strand specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heteroduplexes. J Bacteriol. 1973; 115(3):904-11. PMC: 246335. DOI: 10.1128/jb.115.3.904-911.1973. View

16.

STIRM S, ORSKOV F, ORSKOV I, MANSA B . Episome-carried surface antigen K88 of Escherichia coli. II. Isolation and chemical analysis. J Bacteriol. 1967; 93(2):731-9. PMC: 276502. DOI: 10.1128/jb.93.2.731-739.1967. View

17.

Smith H, Linggood M . Observations on the pathogenic properties of the K88, Hly and Ent plasmids of Escherichia coli with particular reference to porcine diarrhoea. J Med Microbiol. 1971; 4(4):467-85. DOI: 10.1099/00222615-4-4-467. View

18.

STIRM S, ORSKOV F, ORSKOV I, BIRCH-ANDERSEN A . Episome-carried surface antigen K88 of Escherichia coli. 3. Morphology. J Bacteriol. 1967; 93(2):740-8. PMC: 276503. DOI: 10.1128/jb.93.2.740-748.1967. View

19.

ORSKOV I, ORSKOV F . Episome-carried surface antigen K88 of Escherichia coli. I. Transmission of the determinant of the K88 antigen and influence on the transfer of chromosomal markers. J Bacteriol. 1966; 91(1):69-75. PMC: 315911. DOI: 10.1128/jb.91.1.69-75.1966. View

20.

Meyers J, Sanchez D, ELWELL L, Falkow S . Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976; 127(3):1529-37. PMC: 232949. DOI: 10.1128/jb.127.3.1529-1537.1976. View