Genetic and Molecular Characteristics of Vir Plasmids of Bovine Septicemic Escherichia Coli

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1980 Feb 1

PMID 6988390

Citations 5

Authors

J Lopez-Alvarez

C L Gyles

P L Shipley

S Falkow

Affiliations

Soon will be listed here.

Abstract

Three wild strains of bovine septicemic Escherichia coli were selected on the basis of their production of a toxin lethal for mice and chickens and their characteristic surface antigen. The transfer of these virulence (Vir) properties from two of the three to recipient E. coli was detected after mating. One Vir plasmid (pJL1) was derepressed for transfer and associated with mobilization of chromosomal markers. The other, pJL2, was repressed. Both plasmids were tagged with transposon Tn5 (kanamycin resistance), and transfer parameters of the tagged plasmids were studied. The Tn5 insertion in pJL2 usually increased transfer efficiency 100-fold. Plasmid pJL1 was classified as a member of the FIV incompatibility group. A pJL1::Tn5 derivative plasmid was incompatible with ColV1. Plasmid pJL2 behaved as an fi+ plasmid. Both plasmids pJL1 and pJL2 had a molecular weight of 92 x 10(6) and were present at about four copies per chromosome; their deoxyribonucleic acid (DNA) structures were not identical on the basis of restriction enzyme analysis. DNA-DNA hybridization revealed a polynucleotide sequence homology of at least 58% between the two plasmids. No plasmids could be detected in one wild or certain laboratory-derived Vir+ E. coli strains.

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References

Anderson E . A rapid screening test for transfer factors in drug-sensitive Enterobacteriaceae. Nature. 1965; 208(5014):1016-7. DOI: 10.1038/2081016a0. View

Tomoeda M, Inuzuka M, Kubo N, Nakamura S . Effective elimination of drug resistance and sex factors in Escherichia coli by sodium dodecyl sulfate. J Bacteriol. 1968; 95(3):1078-89. PMC: 252134. DOI: 10.1128/jb.95.3.1078-1089.1968. View

Britten R, Kohne D . Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms. Science. 1968; 161(3841):529-40. DOI: 10.1126/science.161.3841.529. View

Marvin D, Hohn B . Filamentous bacterial viruses. Bacteriol Rev. 1969; 33(2):172-209. PMC: 378320. DOI: 10.1128/br.33.2.172-209.1969. View

Smith H, Gyles C . The relationship between two apparently different enterotoxins produced by enteropathogenic strains of Escherichia coli of porcine origin. J Med Microbiol. 1970; 3(3):387-401. DOI: 10.1099/00222615-3-3-387. View

Smith H, Gyles C . The relationship between different transmissible plasmids introduced by F into the same strain of Escherichia coli K12. J Gen Microbiol. 1970; 62(3):227-85. DOI: 10.1099/00221287-62-3-277. View

PITTON J, Anderson E . The inhibitory action of transfer factors on lysis of Escherichia coli K12 by phages mu 2 and phi 2. Genet Res. 1970; 16(2):215-24. DOI: 10.1017/s0016672300002433. View

Grindley J, Anderson E . I-like resistance factors with the fi+ character. Genet Res. 1971; 17(3):267-71. DOI: 10.1017/s0016672300012295. View

Hedges R, Datta N . R124, an fi R factor of a new compatibility class. J Gen Microbiol. 1972; 71(2):403-5. DOI: 10.1099/00221287-71-2-403. View

10.

Cohen S, Chang A, Hsu L . Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A. 1972; 69(8):2110-4. PMC: 426879. DOI: 10.1073/pnas.69.8.2110. View

11.

Truscott R . Studies on the chick-lethal toxin of Eecherichia coli. Can J Comp Med. 1973; 37(4):375-81. PMC: 1319795. View

12.

Turscott R, Lopez-Alvarez J, Pettit J . Studies of Escherichia coli infection in chickens. Can J Comp Med. 1974; 38(2):160-7. PMC: 1319989. View

13.

Zaenen I, Van Larebeke N, Van Montagu M, Schell J . Supercoiled circular DNA in crown-gall inducing Agrobacterium strains. J Mol Biol. 1974; 86(1):109-27. DOI: 10.1016/s0022-2836(74)80011-2. View

14.

Smith H . A search for transmissible pathogenic characters in invasive strains of Escherichia coli: the discovery of a plasmid-controlled toxin and a plasmid-controlled lethal character closely associated, or identical, with colicine V. J Gen Microbiol. 1974; 83(0):95-111. DOI: 10.1099/00221287-83-1-95. View

15.

Falkow S, Guerry P, Hedges R, Datta N . Polynucleotide sequence relationships among plasmids of the I compatibility complex. J Gen Microbiol. 1974; 85(1):65-76. DOI: 10.1099/00221287-85-1-65. View

16.

Crosa J, Luttropp L, Falkow S . Nature of R-factor replication in the presence of chloramphenicol. Proc Natl Acad Sci U S A. 1975; 72(2):654-8. PMC: 432373. DOI: 10.1073/pnas.72.2.654. View

17.

ELWELL L, de Graaff J, Seibert D, Falkow S . Plasmid-linked ampicillin resistance in haempohilus influenza type b. Infect Immun. 1975; 12(2):404-10. PMC: 415297. DOI: 10.1128/iai.12.2.404-410.1975. View

18.

Smith H, Huggins M . Further observations on the association of the colicine V plasmid of Escherichia coli with pathogenicity and with survival in the alimentary tract. J Gen Microbiol. 1976; 92(2):335-50. DOI: 10.1099/00221287-92-2-335. View

19.

Meyers J, Sanchez D, ELWELL L, Falkow S . Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976; 127(3):1529-37. PMC: 232949. DOI: 10.1128/jb.127.3.1529-1537.1976. View

20.

GRANT R, Bannatyne R, Shapiey A . Resistance to chloramphenicol and ampicillin of Salmonella typhimurium in Ontario, Canada. J Infect Dis. 1976; 134(4):354-61. DOI: 10.1093/infdis/134.4.354. View