DLC-1 Facilitates Germ Granule Assembly in Embryo

Overview

Journal Mol Biol Cell

Specialties Cell Biology
Molecular Biology

Date 2022 Mar 11

PMID 35274966

Authors

Nicholas J Day

Mary Ellenbecker

Xiaobo Wang

Ekaterina Voronina

Affiliations

Soon will be listed here.

Abstract

Germ granules are cytoplasmic assemblies of RNA-binding proteins (RBPs) required for germ cell development and fertility. During the first four cell divisions of the zygote, regulated assembly of germ (P) granules leads to their selective segregation to the future germ cell. Here we investigate the role of DLC-1, a hub protein implicated in stabilization and function of diverse protein complexes, in maintaining P granule integrity. We find that DLC-1 directly interacts with several core P granule proteins, predominantly during embryogenesis. The loss of disrupts assembly of P granule components into phase-separated organelles in the embryos, regardless of whether or not DLC-1 directly interacts with these proteins. Finally, we infer that P granule dispersal in the absence of is likely independent of DLC-1's function as a subunit of the dynein motor and does not result from a loss of cell polarity.

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