A Map of the Interactome Network of the Metazoan C. Elegans

Overview

Journal Science

Specialty Science

Date 2004 Jan 6

PMID 14704431

Citations 638

Authors

Siming Li

Christopher M Armstrong

Nicolas Bertin

Hui Ge

Stuart Milstein

Mike Boxem

Pierre-Olivier Vidalain

Jing-Dong J Han

Alban Chesneau

Tong Hao

Debra S Goldberg

Ning Li

Monica Martinez

Jean-Francois Rual

Philippe Lamesch

Lai Xu

Muneesh Tewari

Sharyl L Wong

Lan V Zhang

Gabriel F Berriz

Laurent Jacotot

Philippe Vaglio

Jerome Reboul

Tomoko Hirozane-Kishikawa

Qianru Li

Harrison W Gabel

Ahmed Elewa

Bridget Baumgartner

Debra J Rose

Haiyuan Yu

Stephanie Bosak

Reynaldo Sequerra

Andrew Fraser

Susan E Mango

William M Saxton

Susan Strome

Sander van den Heuvel

Fabio Piano

Jean Vandenhaute

Claude Sardet

Mark Gerstein

Lynn Doucette-Stamm

Kristin C Gunsalus

J Wade Harper

Michael E Cusick

Frederick P Roth

David E Hill

Marc Vidal

Affiliations

Soon will be listed here.

Abstract

To initiate studies on how protein-protein interaction (or "interactome") networks relate to multicellular functions, we have mapped a large fraction of the Caenorhabditis elegans interactome network. Starting with a subset of metazoan-specific proteins, more than 4000 interactions were identified from high-throughput, yeast two-hybrid (HT=Y2H) screens. Independent coaffinity purification assays experimentally validated the overall quality of this Y2H data set. Together with already described Y2H interactions and interologs predicted in silico, the current version of the Worm Interactome (WI5) map contains approximately 5500 interactions. Topological and biological features of this interactome network, as well as its integration with phenome and transcriptome data sets, lead to numerous biological hypotheses.

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