Dielectric Dispersion Modulated Sensing of Yeast Suspension Electroporation

Overview

Journal Sensors (Basel)

Publisher MDPI

Specialty Biotechnology

Date 2022 Mar 10

PMID 35270958

Authors

Guilherme B Pintarelli

Jessica R da Silva

Wuqiang Yang

Daniela O H Suzuki

Affiliations

Soon will be listed here.

Abstract

A specific pulsed electric field protocol can be used to induce electroporation. This is used in the food industry for yeast pasteurization, in laboratories for generic transfer and the medical field for cancer treatment. The sensing of electroporation can be done with simple 'instantaneous' voltage-current analysis. However, there are some intrinsic low-frequency phenomena superposing the electroporation current, such as electrode polarization. The biological media are non-homogeneous, giving them specific characterization in the broad frequency spectrum. For example, the cell barrier, i.e., cell membrane, causes so called β-dispersion in the frequency range of tens to thousands of kHz. Electroporation is a dynamic phenomenon characterized by altering the cell membrane permeability. In this work, we show that the impedance measurement at certain frequencies could be used to detect the occurrence of electroporation, i.e., dielectric dispersion modulated sensing. This approach may be used for the design and implementation of electroporation systems. Yeast suspension electroporation is simulated to show changes in the frequency spectrum. Moreover, the alteration depends on characteristics of the system. Three types of external buffers and their characteristics are evaluated.

Citing Articles

Biological dispersion in the time domain using finite element method software.

Guedert R, Andrade D, Pintarelli G, Suzuki D Sci Rep. 2023; 13(1):22868.

PMID: 38129500 PMC: 10739869. DOI: 10.1038/s41598-023-49828-1.

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