Berry Anthocyanin, Acid, and Volatile Trait Analyses in a Grapevine-Interspecific F2 Population Using an Integrated GBS and RhAmpSeq Genetic Map

Overview

Journal Plants (Basel)

Date 2022 Mar 10

PMID 35270166

Authors

Dilmini Alahakoon

Anne Fennell

Zachary Helget

Terry Bates

Avinash Karn

David Manns

Anna Katharine Mansfield

Bruce I Reisch

Gavin Sacks

Qi Sun

Cheng Zou

Lance Cadle-Davidson

Jason P Londo

Affiliations

Soon will be listed here.

Abstract

Increased map density and transferability of markers are essential for the genetic analysis of fruit quality and stress tolerance in interspecific grapevine populations. We used 1449 GBS and 2000 rhAmpSeq markers to develop a dense map for an interspecific F population (VRS-F) that was derived by selfing a single F from a x 'Seyval blanc' cross. The resultant map contained 2519 markers spanning 1131.3 cM and was highly collinear with the 'PN40024' genome. Quantitative trait loci (QTL) for berry skin color and flower type were used to validate the map. Four rhAmpSeq transferable markers were identified that can be used in pairs (one pistillate and one hermaphroditic) to predict pistillate and hermaphrodite flower type with ≥99.7% accuracy. Total and individual anthocyanin diglucoside QTL mapped to chromosome 9 near a candidate gene. Malic acid QTL were observed on chromosome 1 and 6 with two and () candidate genes, respectively. Modeling malic acid identified a potential QTL on chromosome 8 with peak position in proximity of another ALMT. A first-ever reported QTL for the grassy smelling volatile (E)-2-hexenal was found on chromosome 2 with a candidate gene near peak markers.

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