In Vitro Toxicity of Bone Graft Materials to Human Mineralizing Cells

Overview

Journal Materials (Basel)

Publisher MDPI

Date 2022 Mar 10

PMID 35269185

Authors

Fan Yang

Kao Li

Shi Fu

Michael Cuiffo

Marcia Simon

Miriam Rafailovich

Georgios E Romanos

Affiliations

Soon will be listed here.

Abstract

Bone graft materials from synthetic, bovine, and human sources were analyzed and tested for in vitro cytotoxicity on dental pulp stem cells (DPSCs) and osteosarcoma cells (Saos-2). Raman spectroscopy indicated significant amounts of collagen only in human bone-derived materials, where the mineral to protein ratio was 3.55 ± 0.45, consistent with bone. X-ray fluorescence revealed tungsten (W) concentrations of 463 ± 73, 400 ± 77, and 92 ± 42 ppm in synthetic, bovine, and human bone chips, respectively. When these chips were added to DPSCs on tissue culture plastic, the doubling times after two days were the same as the controls, 16.5 ± 0.5 h. Those cultured with synthetic or bovine chips were 96.5 ± 8.1 and 25.2 ± 1.4 h, respectively. Saos-2 was more sensitive. During the first two days with allogeneic or bovine graft materials, cell numbers declined. When DPSC were cultured on collagen, allogeneic and bovine bone chips did not increase doubling times. We propose cytotoxicity was associated with tungsten, where only the concentration in human bone chips was below 184 ppm, the value reported as cytotoxic in vitro. Cells on collagen were resistant to bone chips, possibly due to tungsten adsorption by collagen.

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References

Lavery T, Kemper C, Sanderson K, Schultz C, Coyle P, Mitchell J . Heavy metal toxicity of kidney and bone tissues in South Australian adult bottlenose dolphins (Tursiops aduncus). Mar Environ Res. 2008; 67(1):1-7. DOI: 10.1016/j.marenvres.2008.09.005. View

Romanos G, Delgado-Ruiz R, Gomez-Moreno G, Lopez-Lopez P, Mate Sanchez de Val J, Calvo-Guirado J . Role of mechanical compression on bone regeneration around a particulate bone graft material: an experimental study in rabbit calvaria. Clin Oral Implants Res. 2015; 29(6):612-619. DOI: 10.1111/clr.12592. View

Grant M, VanderSchee C, Chou H, Bolt A, Epure L, Kuter D . Tungsten accumulates in the intervertebral disc and vertebrae stimulating disc degeneration and upregulating markers of inflammation and pain. Eur Cell Mater. 2021; 41:517-530. DOI: 10.22203/eCM.v041a33. View

Leggett R . A model of the distribution and retention of tungsten in the human body. Sci Total Environ. 1997; 206(2-3):147-65. View

Wang W, Yeung K . Bone grafts and biomaterials substitutes for bone defect repair: A review. Bioact Mater. 2018; 2(4):224-247. PMC: 5935655. DOI: 10.1016/j.bioactmat.2017.05.007. View

Yamada M, Egusa H . Current bone substitutes for implant dentistry. J Prosthodont Res. 2017; 62(2):152-161. DOI: 10.1016/j.jpor.2017.08.010. View

Guandalini G, Zhang L, Fornero E, Centeno J, Mokashi V, Ortiz P . Tissue distribution of tungsten in mice following oral exposure to sodium tungstate. Chem Res Toxicol. 2011; 24(4):488-93. DOI: 10.1021/tx200011k. View

Kelly A, Lemaire M, Young Y, Eustache J, Guilbert C, Flores Molina M . In vivo tungsten exposure alters B-cell development and increases DNA damage in murine bone marrow. Toxicol Sci. 2012; 131(2):434-46. DOI: 10.1093/toxsci/kfs324. View

Sallent I, Capella-Monsonis H, Procter P, Bozo I, Deev R, Zubov D . The Few Who Made It: Commercially and Clinically Successful Innovative Bone Grafts. Front Bioeng Biotechnol. 2020; 8:952. PMC: 7490292. DOI: 10.3389/fbioe.2020.00952. View

10.

Aghaloo T, Moy P . Which hard tissue augmentation techniques are the most successful in furnishing bony support for implant placement?. Int J Oral Maxillofac Implants. 2008; 22 Suppl:49-70. View

11.

Romanos G . Anatomical and Biologic Considerations of Autogenous Bone Blocks Harvested from the Ramus Region. Int J Oral Maxillofac Implants. 2018; 34(1):e1–e6. DOI: 10.11607/jomi.7069. View

12.

Delgado-Ruiz R, Calvo Guirado J, Romanos G . Bone grafting materials in critical defects in rabbit calvariae. A systematic review and quality evaluation using ARRIVE guidelines. Clin Oral Implants Res. 2015; 29(6):620-634. DOI: 10.1111/clr.12614. View

13.

Bibr B, Deyl Z, Lener J, Kucera J, Simkova M . The mechanism of action of molybdenum and tungsten upon collagen structures in vivo. Physiol Bohemoslov. 1987; 36(5):417-24. View

14.

Alliot-Licht B, Bluteau G, Magne D, Lopez-Cazaux S, Lieubeau B, Daculsi G . Dexamethasone stimulates differentiation of odontoblast-like cells in human dental pulp cultures. Cell Tissue Res. 2005; 321(3):391-400. DOI: 10.1007/s00441-005-1115-7. View

15.

van der Voet G, Todorov T, Centeno J, Jonas W, Ives J, Mullick F . Metals and health: a clinical toxicological perspective on tungsten and review of the literature. Mil Med. 2007; 172(9):1002-5. DOI: 10.7205/milmed.172.9.1002. View

16.

Delgado-Ruiz R, Romanos G, Alexandre Gerhke S, Gomez-Moreno G, Mate-Sanchez de Val J, Calvo-Guirado J . Biological effects of compressive forces exerted on particulate bone grafts during socket preservation: animal study. Clin Oral Implants Res. 2016; 29(7):792-801. DOI: 10.1111/clr.12942. View

17.

Gentleman E, Swain R, Evans N, Boonrungsiman S, Jell G, Ball M . Comparative materials differences revealed in engineered bone as a function of cell-specific differentiation. Nat Mater. 2009; 8(9):763-70. DOI: 10.1038/nmat2505. View

18.

Perugini M, Visciano P, Manera M, Zaccaroni A, Olivieri V, Amorena M . Heavy metal (As, Cd, Hg, Pb, Cu, Zn, Se) concentrations in muscle and bone of four commercial fish caught in the central Adriatic Sea, Italy. Environ Monit Assess. 2013; 186(4):2205-13. DOI: 10.1007/s10661-013-3530-7. View

19.

Osterburg A, Robinson C, Schwemberger S, Mokashi V, Stockelman M, Babcock G . Sodium tungstate (Na2WO4) exposure increases apoptosis in human peripheral blood lymphocytes. J Immunotoxicol. 2010; 7(3):174-82. DOI: 10.3109/15476911003631617. View