Human Parathyroid Antigen: Characterization and Localization with Monoclonal Antibodies

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1986 Aug 1

PMID 3526352

Citations 2

Authors

W G Cance

S A Wells Jr

W G Dilley

M J Welch

F L Otsuka

J M Davie

Affiliations

Soon will be listed here.

Abstract

A cell surface antigen on human parathyroid cells was identified by monoclonal antibodies. The antigen, called parathyroid antigen (PTA), is found on both of two major polypeptides (190 kDa and 160 kDa) apparently associated exclusively with parathyroid cells. To determine whether PTA was a suitable target for in vivo imaging, 125I-labeled anti-PTA was injected into nude mice bearing human parathyroid xenografts. IgG1 anti-PTA antibody showed excellent radiolocalization of the grafts, whereas IgM anti-PTA, containing the same variable domains as the IgG1 antibody, showed little specific binding. These results suggest that antibody isotype is an important parameter for the in vivo immunoscintigraphy of specific cellular antigens.

Citing Articles

Monoclonal antibodies with exclusive reactivity against parathyroid cells and tubule cells of the kidney.

Juhlin C, Holmdahl R, Johansson H, Rastad J, Akerstrom G, Klareskog L Proc Natl Acad Sci U S A. 1987; 84(9):2990-4.

PMID: 3554250 PMC: 304786. DOI: 10.1073/pnas.84.9.2990.

Monoclonal antiparathyroid antibodies revealing defect expression of a calcium receptor mechanism in hyperparathyroidism.

Juhlin C, Akerstrom G, Klareskog L, Gylfe E, Johansson H, Larsson R World J Surg. 1988; 12(4):552-8.

PMID: 2844028 DOI: 10.1007/BF01655449.

References

Pressman D, Korngold L . The in vivo localization of anti-Wagner-osteogenic-sarcoma antibodies. Cancer. 1953; 6(3):619-23. DOI: 10.1002/1097-0142(195305)6:3<619::aid-cncr2820060319>3.0.co;2-y. View

Dangl J, Herzenberg L . Selection of hybridomas and hybridoma variants using the fluorescence activated cell sorter. J Immunol Methods. 1982; 52(1):1-14. DOI: 10.1016/0022-1759(82)90344-1. View

Cohn D, Morrissey J, Hamilton J, Shofstall R, Smardo F, Chu L . Isolation and partial characterization of secretory protein I from bovine parathyroid glands. Biochemistry. 1981; 20(14):4135-40. DOI: 10.1021/bi00517a029. View

Pimm M, Baldwin R . Distribution of IgM monoclonal antibody in mice with human tumour xenografts: lack of tumour localization. Eur J Cancer Clin Oncol. 1985; 21(6):765-8. DOI: 10.1016/0277-5379(85)90276-7. View

Radbruch A, Liesegang B, Rajewsky K . Isolation of variants of mouse myeloma X63 that express changed immunoglobulin class. Proc Natl Acad Sci U S A. 1980; 77(5):2909-13. PMC: 349515. DOI: 10.1073/pnas.77.5.2909. View

Midoux P, Maillet T, Therain F, Monsigny M, Roche A . Tumor localization of Lewis lung carcinoma with radiolabeled monoclonal antibodies. Cancer Immunol Immunother. 1984; 18(1):19-23. PMC: 11039291. DOI: 10.1007/BF00205394. View

Markwell M, Fox C . Surface-specific iodination of membrane proteins of viruses and eucaryotic cells using 1,3,4,6-tetrachloro-3alpha,6alpha-diphenylglycoluril. Biochemistry. 1978; 17(22):4807-17. DOI: 10.1021/bi00615a031. View

Kipps T, Parham P, Punt J, Herzenberg L . Importance of immunoglobulin isotype in human antibody-dependent, cell-mediated cytotoxicity directed by murine monoclonal antibodies. J Exp Med. 1985; 161(1):1-17. PMC: 2187540. DOI: 10.1084/jem.161.1.1. View

Zimmer A, Rosen S, Spies S, Polovina M, Minna J, Spies W . Radioimmunoimaging of human small cell lung carcinoma with I-131 tumor specific monoclonal antibody. Hybridoma. 1985; 4(1):1-11. DOI: 10.1089/hyb.1985.4.1. View

10.

Brown E, Hurwitz S, Aurbach G . Preparation of viable isolated bovine parathyroid cells. Endocrinology. 1976; 99(6):1582-8. DOI: 10.1210/endo-99-6-1582. View

11.

Pimm M, Armitage N, Perkins A, Smith W, Baldwin R . Localization of an anti-CEA monoclonal antibody in colo-rectal carcinoma xenografts. Cancer Immunol Immunother. 1985; 19(1):8-17. PMC: 11039282. DOI: 10.1007/BF00199305. View

12.

Cohn D, ELTING J . Biosynthesis, processing, and secretion of parathormone and secretory protein-I. Recent Prog Horm Res. 1983; 39:181-209. DOI: 10.1016/b978-0-12-571139-5.50008-2. View

13.

Martorelli Jr B . Effects of chemotherapeutic agents on two lines of human breast carcinomas in tissue culture. Surg Gynecol Obstet. 1969; 128(5):1001-6. View

14.

Mann B, Cohen M, Saxton R, Morton D, Benedict W, Korn E . Imaging of human tumor xenografts in nude mice with radiolabeled monoclonal antibodies. Limitations of specificity due to nonspecific uptake of antibody. Cancer. 1984; 54(7):1318-27. DOI: 10.1002/1097-0142(19841001)54:7<1318::aid-cncr2820540715>3.0.co;2-j. View

15.

Scheinberg D, Strand M, Gansow O . Tumor imaging with radioactive metal chelates conjugated to monoclonal antibodies. Science. 1982; 215(4539):1511-3. DOI: 10.1126/science.7199757. View

16.

Fraker P, SPECK Jr J . Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphrenylglycoluril. Biochem Biophys Res Commun. 1978; 80(4):849-57. DOI: 10.1016/0006-291x(78)91322-0. View

17.

Schlom J, Greiner J, HAND P, Colcher D, Inghirami G, Weeks M . Monoclonal antibodies to breast cancer-associated antigens as potential reagents in the management of breast cancer. Cancer. 1984; 54(11 Suppl):2777-94. DOI: 10.1002/1097-0142(19841201)54:2+<2777::aid-cncr2820541426>3.0.co;2-d. View

18.

VOISIN G . Studies in vascular permeability. II. Comparative extravasation of different immunoglobulin classes in normal guinea pig skin. Eur J Immunol. 1973; 3(11):663-7. DOI: 10.1002/eji.1830031102. View

19.

Yelton D, Desaymard C, Scharff M . Use of monoclonal anti-mouse immunoglobulin to detect mouse antibodies. Hybridoma. 1981; 1(1):5-11. DOI: 10.1089/hyb.1.1981.1.5. View

20.

Steplewski Z, Spira G, Blaszczyk M, Lubeck M, Radbruch A, Illges H . Isolation and characterization of anti-monosialoganglioside monoclonal antibody 19-9 class-switch variants. Proc Natl Acad Sci U S A. 1985; 82(24):8653-7. PMC: 391494. DOI: 10.1073/pnas.82.24.8653. View