Use of Monoclonal Anti-mouse Immunoglobulin to Detect Mouse Antibodies

Overview

Journal Hybridoma

Publisher Mary Ann Liebert

Specialty Cell Biology

Date 1981 Jan 1

PMID 6821395

Citations 79

Authors

D E Yelton

C Desaymard

M D Scharff

Affiliations

Soon will be listed here.

Abstract

Rat monoclonal antibodies specific for mouse kappa light chains and mouse gamma heavy chains have been generated. These rat monoclonal antibodies have been biosynthetically labelled with 35S methionine. The free label was dialyzed from the medium and, without further purification, the medium containing the radioactive monoclonal antibody was used in a radioimmunoassay to screen the sera of the immunized animals and hybridomas for specific mouse antibodies of the IgG class.

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