Exploring Cocoa Bean Fermentation Mechanisms by Kinetic Modelling

Overview

Journal R Soc Open Sci

Specialty Science

Date 2022 Feb 28

PMID 35223050

Authors

Mauricio Moreno-Zambrano

Matthias S Ullrich

Marc-Thorsten Hutt

Affiliations

Soon will be listed here.

Abstract

Compared with other fermentation processes in food industry, cocoa bean fermentation is uncontrolled and not standardized. A detailed mechanistic understanding can therefore be relevant for cocoa bean quality control. Starting from an existing mathematical model of cocoa bean fermentation we analyse five additional biochemical mechanisms derived from the literature. These mechanisms, when added to the baseline model either in isolation or in combination, were evaluated in terms of their capacity to describe experimental data. In total, we evaluated 32 model variants on 23 fermentation datasets. We interpret the results from two perspectives: (1) success of the potential mechanism, (2) discrimination of fermentation protocols based on estimated parameters. The former provides insight in the fermentation process itself. The latter opens an avenue towards reverse-engineering empirical conditions from model parameters. We find support for two mechanisms debated in the literature: consumption of fructose by lactic acid bacteria and production of acetic acid by yeast. Furthermore, we provide evidence that model parameters are sensitive to differences in the cultivar, temperature control and usage of steel tanks compared with wooden boxes. Our results show that mathematical modelling can provide an alternative to standard chemical fingerprinting in the interpretation of fermentation data.

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References

Camu N, De Winter T, Verbrugghe K, Cleenwerck I, Vandamme P, Takrama J . Dynamics and biodiversity of populations of lactic acid bacteria and acetic acid bacteria involved in spontaneous heap fermentation of cocoa beans in Ghana. Appl Environ Microbiol. 2007; 73(6):1809-24. PMC: 1828797. DOI: 10.1128/AEM.02189-06. View

Camu N, Gonzalez A, De Winter T, Van Schoor A, De Bruyne K, Vandamme P . Influence of turning and environmental contamination on the dynamics of populations of lactic acid and acetic acid bacteria involved in spontaneous cocoa bean heap fermentation in Ghana. Appl Environ Microbiol. 2007; 74(1):86-98. PMC: 2223199. DOI: 10.1128/AEM.01512-07. View

Schwan R, Wheals A . The microbiology of cocoa fermentation and its role in chocolate quality. Crit Rev Food Sci Nutr. 2004; 44(4):205-21. DOI: 10.1080/10408690490464104. View

de Melo Pereira G, Magalhaes K, Gonzaga de Almeida E, da Silva Coelho I, Schwan R . Spontaneous cocoa bean fermentation carried out in a novel-design stainless steel tank: influence on the dynamics of microbial populations and physical-chemical properties. Int J Food Microbiol. 2013; 161(2):121-33. DOI: 10.1016/j.ijfoodmicro.2012.11.018. View

Romanens E, Naf R, Lobmaier T, Pedan V, Freimuller Leischtfeld S, Meile L . A lab-scale model system for cocoa bean fermentation. Appl Microbiol Biotechnol. 2018; 102(7):3349-3362. DOI: 10.1007/s00253-018-8835-6. View

Papalexandratou Z, Vrancken G, De Bruyne K, Vandamme P, De Vuyst L . Spontaneous organic cocoa bean box fermentations in Brazil are characterized by a restricted species diversity of lactic acid bacteria and acetic acid bacteria. Food Microbiol. 2011; 28(7):1326-38. DOI: 10.1016/j.fm.2011.06.003. View

Watson H . A Note on the Variation of the Rate of Disinfection with Change in the Concentration of the Disinfectant. J Hyg (Lond). 2010; 8(4):536-42. PMC: 2167149. DOI: 10.1017/s0022172400015928. View

De Vuyst L, Leroy F . Functional role of yeasts, lactic acid bacteria and acetic acid bacteria in cocoa fermentation processes. FEMS Microbiol Rev. 2020; 44(4):432-453. DOI: 10.1093/femsre/fuaa014. View

Racine K, Lee A, Wiersema B, Huang H, Lambert J, Stewart A . Development and Characterization of a Pilot-Scale Model Cocoa Fermentation System Suitable for Studying the Impact of Fermentation on Putative Bioactive Compounds and Bioactivity of Cocoa. Foods. 2019; 8(3). PMC: 6463099. DOI: 10.3390/foods8030102. View

10.

Figueroa-Hernandez C, Mota-Gutierrez J, Ferrocino I, Hernandez-Estrada Z, Gonzalez-Rios O, Cocolin L . The challenges and perspectives of the selection of starter cultures for fermented cocoa beans. Int J Food Microbiol. 2019; 301:41-50. DOI: 10.1016/j.ijfoodmicro.2019.05.002. View

11.

Lefeber T, Janssens M, Camu N, De Vuyst L . Kinetic analysis of strains of lactic acid bacteria and acetic acid bacteria in cocoa pulp simulation media toward development of a starter culture for cocoa bean fermentation. Appl Environ Microbiol. 2010; 76(23):7708-16. PMC: 2988588. DOI: 10.1128/AEM.01206-10. View

12.

Carpenter B, Gelman A, Hoffman M, Lee D, Goodrich B, Betancourt M . Stan: A Probabilistic Programming Language. J Stat Softw. 2022; 76. PMC: 9788645. DOI: 10.18637/jss.v076.i01. View

13.

Papalexandratou Z, Falony G, Romanens E, Jimenez J, Amores F, Daniel H . Species diversity, community dynamics, and metabolite kinetics of the microbiota associated with traditional ecuadorian spontaneous cocoa bean fermentations. Appl Environ Microbiol. 2011; 77(21):7698-714. PMC: 3209185. DOI: 10.1128/AEM.05523-11. View

14.

Lefeber T, Gobert W, Vrancken G, Camu N, De Vuyst L . Dynamics and species diversity of communities of lactic acid bacteria and acetic acid bacteria during spontaneous cocoa bean fermentation in vessels. Food Microbiol. 2011; 28(3):457-64. DOI: 10.1016/j.fm.2010.10.010. View

15.

Viesser J, Pereira G, de Carvalho Neto D, Vandenberghe L, Azevedo V, Brenig B . Exploring the contribution of fructophilic lactic acid bacteria to cocoa beans fermentation: Isolation, selection and evaluation. Food Res Int. 2020; 136:109478. DOI: 10.1016/j.foodres.2020.109478. View

16.

Caligiani A, Palla L, Acquotti D, Marseglia A, Palla G . Application of 1H NMR for the characterisation of cocoa beans of different geographical origins and fermentation levels. Food Chem. 2014; 157:94-9. DOI: 10.1016/j.foodchem.2014.01.116. View

17.

Lagunes Galvez S, Loiseau G, Paredes J, Barel M, Guiraud J . Study on the microflora and biochemistry of cocoa fermentation in the Dominican Republic. Int J Food Microbiol. 2006; 114(1):124-30. DOI: 10.1016/j.ijfoodmicro.2006.10.041. View

18.

Bastos V, Santos M, Gomes L, Leite A, Paschoalin V, Del Aguila E . Analysis of the cocobiota and metabolites of Moniliophthora perniciosa-resistant Theobroma cacao beans during spontaneous fermentation in southern Brazil. J Sci Food Agric. 2018; 98(13):4963-4970. DOI: 10.1002/jsfa.9029. View

19.

Lefeber T, Papalexandratou Z, Gobert W, Camu N, De Vuyst L . On-farm implementation of a starter culture for improved cocoa bean fermentation and its influence on the flavour of chocolates produced thereof. Food Microbiol. 2012; 30(2):379-92. DOI: 10.1016/j.fm.2011.12.021. View

20.

John W, Bottcher N, Behrends B, Corno M, Dsouza R, Kuhnert N . Experimentally modelling cocoa bean fermentation reveals key factors and their influences. Food Chem. 2019; 302:125335. DOI: 10.1016/j.foodchem.2019.125335. View