Histone H2B Deacylation Selectivity: Exploring Chromatin's Dark Matter with an Engineered Sortase

Overview

Journal J Am Chem Soc

Publisher American Chemical Society

Specialty Chemistry

Date 2022 Feb 17

PMID 35175758

Authors

Zhipeng A Wang

Samuel D Whedon

Mingxuan Wu

Siyu Wang

Edward A Brown

Ananya Anmangandla

Liam Regan

Kwangwoon Lee

Jianfeng Du

Jun Young Hong

Louise Fairall

Taylor Kay

Hening Lin

Yingming Zhao

John W R Schwabe

Philip A Cole

Affiliations

Soon will be listed here.

Abstract

We describe a new method to produce histone H2B by semisynthesis with an engineered sortase transpeptidase. N-Terminal tail site-specifically modified acetylated, lactylated, and β-hydroxybutyrylated histone H2Bs were incorporated into nucleosomes and investigated as substrates of histone deacetylase (HDAC) complexes and sirtuins. A wide range of rates and site-specificities were observed by these enzyme forms suggesting distinct biological roles in regulating chromatin structure and epigenetics.

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