Diversity of Site-specific Microbes of Occlusal and Proximal Lesions in Severe- Early Childhood Caries (S-ECC)

Overview

Journal J Oral Microbiol

Specialty Dentistry

Date 2022 Feb 17

PMID 35173909

Authors

Kausar Sadia Fakhruddin

Lakshman Perera Samaranayake

Rifat Akram Hamoudi

Hien Chi Ngo

Hiroshi Egusa

Affiliations

Soon will be listed here.

Abstract

Background: Severe-early childhood caries (S-ECC) a global problem of significant concern, commonly manifest on the occlusal, and proximal surfaces of affected teeth. Despite the major ecological differences between these two niches the compositional differences, if any, in the microbiota of such lesions is unknown.

Methods: Deep-dentine caries samples from asymptomatic primary molars of children with S-ECC (n 19) belonging to caries-code 5/6, (ICDAS classification) were evaluated. Employing two primer pools, we amplified and compared the bacterial gene sequences of the seven hypervariable regions (V2-V4 and V6-V9) using NGS-based assay.

Results: Bray-Curtisevaluation indicated that occlusal lesions (OL) had a more homogeneous community than the proximal lesions (PL) with significant compositional differences at the species level ( = 0.01; R- 0.513). Together, the occlusal and proximal niches harbored 263 species, of which 202 (76.8%) species were common to both , while 49 (18.6%) and 12 (4.6%) disparate species were exclusively isolated from the proximal and occlusal niches, respectively. The most commonl genera at both niches included , and was predominant in PL ( ≤ 0.05), and ( = 0.01) was predominant in OL.

Conclusions: Distinct differences exist between the caries microbiota of occlusal and proximal caries in S-ECC.

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