A Self-immolative Linker That Releases Thiols Detects Penicillin Amidase and Nitroreductase with High Sensitivity Absorption Spectroscopy

Overview

Journal Chem Commun (Camb)

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2022 Feb 16

PMID 35170593

Authors

Eli M Espinoza

Joachim J Roise

Maomao He

I-Che Li

Alvin K Agatep

Patrick Udenyi

Hesong Han

Nicole Jackson

D Lucas Kerr

Dake Chen

Michael R Stentzel

Emily Ruan

Lee Riley

Niren Murthy

Affiliations

Soon will be listed here.

Abstract

This article reports the synthesis and characterization of a novel self-immolative linker, based on thiocarbonates, which releases a free thiol upon activation enzymes. We demonstrate that thiocarbonate self-immolative linkers can be used to detect the enzymes penicillin G amidase (PGA) and nitroreductase (NTR) with high sensitivity using absorption spectroscopy. Paired with modern thiol amplification technology, the detection of PGA and NTR were achieved at concentrations of 160 nM and 52 nM respectively. In addition, the PGA probe was shown to be compatible with both biological thiols and enzymes present in cell lysates.

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