An Enzyme-Mediated Amplification Strategy Enables Detection of β-Lactamase Activity Directly in Unprocessed Clinical Samples for Phenotypic Detection of β-Lactam Resistance

Overview

Journal Chembiochem

Specialty Biochemistry

Date 2018 Aug 7

PMID 30079487

Citations 9

Authors

Tara R deBoer

Nicole J Tarlton

Reina Yamaji

Sheila Adams-Sapper

Tiffany Z Wu

Santanu Maity

Giri K Vesgesna

Corinne M Sadlowski

Peter DePaola 4th

Lee W Riley

Niren Murthy

Affiliations

Soon will be listed here.

Abstract

Biochemical assays that can identify β-lactamase activity directly from patient samples have the potential to significantly improve the treatment of bacterial infections. However, current β-lactamase probes do not have the sensitivity needed to measure β-lactam resistance directly from patient samples. Here, we report the development of an instrument-free signal amplification technology, DETECT, that connects the activity of two enzymes in series to effectively amplify the activity of β-lactamase 40 000-fold, compared to the standard β-lactamase probe nitrocefin.

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A rapid, antibiotic susceptibility test for multidrug-resistant, Gram-negative bacterial uropathogens using the biochemical assay, DETECT.

Jackson N, Borges C, Tarlton N, Resendez A, Milton A, de Boer T J Microbiol Methods. 2021; 182:106160.

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References

Adams-Sapper S, Nolen S, Donzelli G, Lal M, Chen K, Justo da Silva L . Rapid induction of high-level carbapenem resistance in heteroresistant KPC-producing Klebsiella pneumoniae. Antimicrob Agents Chemother. 2015; 59(6):3281-9. PMC: 4432212. DOI: 10.1128/AAC.05100-14. View

Dallenne C, da Costa A, Decre D, Favier C, Arlet G . Development of a set of multiplex PCR assays for the detection of genes encoding important beta-lactamases in Enterobacteriaceae. J Antimicrob Chemother. 2010; 65(3):490-5. DOI: 10.1093/jac/dkp498. View

Galar A, Leiva J, Espinosa M, Guillen-Grima F, Hernaez S, Yuste J . Clinical and economic evaluation of the impact of rapid microbiological diagnostic testing. J Infect. 2012; 65(4):302-9. DOI: 10.1016/j.jinf.2012.06.006. View

Blaschke A, Hersh A, Beekmann S, Ince D, Polgreen P, Hanson K . Unmet diagnostic needs in infectious disease. Diagn Microbiol Infect Dis. 2014; 81(1):57-9. PMC: 4268087. DOI: 10.1016/j.diagmicrobio.2014.10.005. View

Tarlton N, Satoorian T, Panchal A, Borges C, Geisberg M, Riley L . Monoclonal antibody-mediated detection of CTX-M β-lactamases in Gram-negative bacteria. J Microbiol Methods. 2017; 144:37-43. DOI: 10.1016/j.mimet.2017.09.017. View

Perez K, Olsen R, Musick W, Cernoch P, Davis J, Peterson L . Integrating rapid diagnostics and antimicrobial stewardship improves outcomes in patients with antibiotic-resistant Gram-negative bacteremia. J Infect. 2014; 69(3):216-25. DOI: 10.1016/j.jinf.2014.05.005. View

Sugden R, Kelly R, Davies S . Combatting antimicrobial resistance globally. Nat Microbiol. 2016; 1(10):16187. DOI: 10.1038/nmicrobiol.2016.187. View

Singh R, Blattler W, Collinson A . An amplified assay for thiols based on reactivation of papain. Anal Biochem. 1993; 213(1):49-56. DOI: 10.1006/abio.1993.1384. View

OCALLAGHAN C, Morris A, Kirby S, Shingler A . Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate. Antimicrob Agents Chemother. 1972; 1(4):283-8. PMC: 444209. DOI: 10.1128/AAC.1.4.283. View

10.

Rudresh S, Ravi G, Sunitha L, Hajira S, Kalaiarasan E, Harish B . Simple, rapid, and cost-effective modified Carba NP test for carbapenemase detection among Gram-negative bacteria. J Lab Physicians. 2017; 9(4):303-307. PMC: 5607762. DOI: 10.4103/JLP.JLP_138_16. View

11.

Smith S, Manges A, Riley L . Temporal changes in the prevalence of community-acquired antimicrobial-resistant urinary tract infection affected by Escherichia coli clonal group composition. Clin Infect Dis. 2008; 46(5):689-95. DOI: 10.1086/527386. View

12.

Adams-Sapper S, Diep B, Perdreau-Remington F, Riley L . Clonal composition and community clustering of drug-susceptible and -resistant Escherichia coli isolates from bloodstream infections. Antimicrob Agents Chemother. 2012; 57(1):490-7. PMC: 3535975. DOI: 10.1128/AAC.01025-12. View

13.

Renvoise A, Decre D, Amarsy-Guerle R, Huang T, Jost C, Podglajen I . Evaluation of the βLacta test, a rapid test detecting resistance to third-generation cephalosporins in clinical strains of Enterobacteriaceae. J Clin Microbiol. 2013; 51(12):4012-7. PMC: 3838025. DOI: 10.1128/JCM.01936-13. View

14.

Brocklehurst K, Little G . Reactions of papain and of low-molecular-weight thiols with some aromatic disulphides. 2,2'-Dipyridyl disulphide as a convenient active-site titrant for papain even in the presence of other thiols. Biochem J. 1973; 133(1):67-80. PMC: 1177671. DOI: 10.1042/bj1330067. View

15.

Yamaji R, Rubin J, Thys E, Friedman C, Riley L . Persistent Pandemic Lineages of Uropathogenic Escherichia coli in a College Community from 1999 to 2017. J Clin Microbiol. 2018; 56(4). PMC: 5869836. DOI: 10.1128/JCM.01834-17. View

16.

Shinde S, Gupta R, Raut S, Nataraj G, Mehta P . Carba NP as a simpler, rapid, cost-effective, and a more sensitive alternative to other phenotypic tests for detection of carbapenem resistance in routine diagnostic laboratories. J Lab Physicians. 2017; 9(2):100-103. PMC: 5320871. DOI: 10.4103/0974-2727.199628. View