Cryo-Treatment Enhances the Embryogenicity of Mature Somatic Embryos Via the LncRNA-miRNA-mRNA Network in White Spruce

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2022 Feb 15

PMID 35163033

Authors

Ying Gao

Ying Cui

Ruirui Zhao

Xiaoyi Chen

Jinfeng Zhang

Jian Zhao

Lisheng Kong

Affiliations

Soon will be listed here.

Abstract

In conifers, somatic embryogenesis is uniquely initiated from immature embryos in a narrow time window, which is considerably hindered by the difficulty to induce embryogenic tissue (ET) from other tissues, including mature somatic embryos. In this study, the embryogenic ability of newly induced ET and DNA methylation levels was detected, and whole-transcriptome sequencing analyses were carried out. The results showed that ultra-low temperature treatment significantly enhanced ET induction from mature somatic embryos, with the induction rate from 0.4% to 15.5%, but the underlying mechanisms remain unclear. The newly induced ET showed higher capability in generating mature embryos than the original ET. DNA methylation levels fluctuated during the ET induction process. Here, WGCNA analysis revealed that and genes are involved in stress response and auxin signal transduction. Through co-expression analysis, lncRNAs MSTRG.505746.1, MSTRG.1070680.1, and MSTRG.33602.1 might bind to pre-novel_miR_339 to promote the expression of genes for stress response; could be protected by lncRNAs MSTRG.1070680.1 and MSTRG.33602.1 via serving as sponges for novel_miR_495 to initiate auxin signal transduction; lncRNAs MSTRG.505746.1, MSTRG.1070680.1, and MSTRG.33602.1 might serve as sponges for novel_miR_527 to enhance the expression of for early somatic embryo development. This study provides new insight into the area of stress-enhanced early somatic embryogenesis in conifers, which is also attributable to practical applications.

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References

Li J, Ma W, Zeng P, Wang J, Geng B, Yang J . LncTar: a tool for predicting the RNA targets of long noncoding RNAs. Brief Bioinform. 2014; 16(5):806-12. DOI: 10.1093/bib/bbu048. View

Shen C, Bai Y, Wang S, Zhang S, Wu Y, Chen M . Expression profile of PIN, AUX/LAX and PGP auxin transporter gene families in Sorghum bicolor under phytohormone and abiotic stress. FEBS J. 2010; 277(14):2954-69. DOI: 10.1111/j.1742-4658.2010.07706.x. View

Bustin S, Benes V, Garson J, Hellemans J, Huggett J, Kubista M . The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem. 2009; 55(4):611-22. DOI: 10.1373/clinchem.2008.112797. View

Litvay J, Verma D, Johnson M . Influence of a loblolly pine (Pinus taeda L.). Culture medium and its components on growth and somatic embryogenesis of the wild carrot (Daucus carota L.). Plant Cell Rep. 2013; 4(6):325-8. DOI: 10.1007/BF00269890. View

Pan W, Tao J, Cheng T, Bian X, Wei W, Zhang W . Soybean miR172a Improves Salt Tolerance and Can Function as a Long-Distance Signal. Mol Plant. 2016; 9(9):1337-1340. DOI: 10.1016/j.molp.2016.05.010. View

Vondrakova Z, Cvikrova M, Eliasova K, Martincova O, Vagner M . Cryotolerance in Norway spruce and its association with growth rates, anatomical features and polyamines of embryogenic cultures. Tree Physiol. 2010; 30(10):1335-48. DOI: 10.1093/treephys/tpq074. View

Hoyerova K, Perry L, Hand P, Lankova M, Kocabek T, May S . Functional characterization of PaLAX1, a putative auxin permease, in heterologous plant systems. Plant Physiol. 2008; 146(3):1128-41. PMC: 2259084. DOI: 10.1104/pp.107.109371. View

Hu X, Zhou S, Yang Y, Liu H, Anil S, Wang Q . Transcriptome-wide identification and profiling of miRNAs in a stress-tolerant conifer . J Biosci. 2020; 45. View

Lease K, Lau N, Schuster R, Torii K, Walker J . Receptor serine/threonine protein kinases in signalling: analysis of the erecta receptor-like kinase of Arabidopsis thaliana. New Phytol. 2021; 151(1):133-143. DOI: 10.1046/j.1469-8137.2001.00150.x. View

10.

Wu X, Liu M, Ge X, Xu Q, Guo W . Stage and tissue-specific modulation of ten conserved miRNAs and their targets during somatic embryogenesis of Valencia sweet orange. Planta. 2010; 233(3):495-505. DOI: 10.1007/s00425-010-1312-9. View

11.

Zhang J, Wu T, Li L, Han S, Li X, Zhang S . Dynamic expression of small RNA populations in larch (Larix leptolepis). Planta. 2012; 237(1):89-101. DOI: 10.1007/s00425-012-1753-4. View

12.

Zhou X, Cui J, Meng J, Luan Y . Interactions and links among the noncoding RNAs in plants under stresses. Theor Appl Genet. 2020; 133(12):3235-3248. DOI: 10.1007/s00122-020-03690-1. View

13.

Park Y, Pond S, Bonga J . Somatic embryogenesis in white spruce (Picea glauca): genetic control in somatic embryos exposed to storage, maturation treatments, germination, and cryopreservation. Theor Appl Genet. 2013; 89(6):742-50. DOI: 10.1007/BF00223714. View

14.

Khodakovskaya M, Sword C, Wu Q, Perera I, Boss W, Brown C . Increasing inositol (1,4,5)-trisphosphate metabolism affects drought tolerance, carbohydrate metabolism and phosphate-sensitive biomass increases in tomato. Plant Biotechnol J. 2009; 8(2):170-83. DOI: 10.1111/j.1467-7652.2009.00472.x. View

15.

Langmead B, Trapnell C, Pop M, Salzberg S . Ultrafast and memory-efficient alignment of short DNA sequences to the human genome. Genome Biol. 2009; 10(3):R25. PMC: 2690996. DOI: 10.1186/gb-2009-10-3-r25. View

16.

Su C, Wang Y, Hsieh T, Lu C, Tseng T, Yu S . A novel MYBS3-dependent pathway confers cold tolerance in rice. Plant Physiol. 2010; 153(1):145-58. PMC: 2862423. DOI: 10.1104/pp.110.153015. View

17.

Ariel F, Romero-Barrios N, Jegu T, Benhamed M, Crespi M . Battles and hijacks: noncoding transcription in plants. Trends Plant Sci. 2015; 20(6):362-71. DOI: 10.1016/j.tplants.2015.03.003. View

18.

Varhanikova M, Uvackova L, Skultety L, Pretova A, Obert B, Hajduch M . Comparative quantitative proteomic analysis of embryogenic and non-embryogenic calli in maize suggests the role of oxylipins in plant totipotency. J Proteomics. 2014; 104:57-65. DOI: 10.1016/j.jprot.2014.02.003. View

19.

Yakovlev I, Fossdal C, Johnsen O . MicroRNAs, the epigenetic memory and climatic adaptation in Norway spruce. New Phytol. 2010; 187(4):1154-1169. DOI: 10.1111/j.1469-8137.2010.03341.x. View

20.

Afzal A, Wood A, Lightfoot D . Plant receptor-like serine threonine kinases: roles in signaling and plant defense. Mol Plant Microbe Interact. 2008; 21(5):507-17. DOI: 10.1094/MPMI-21-5-0507. View