Green Extraction of Carrageenans from

Overview

Journal Polymers (Basel)

Publisher MDPI

Date 2022 Feb 15

PMID 35160543

Authors

Noelia Florez-Fernandez

Elena Falque

Herminia Dominguez

Maria Dolores Torres

Affiliations

Soon will be listed here.

Abstract

The recovery of biopolymers from natural resources using eco-friendly extraction technologies that enhance their mechanical properties has gained attention in recent years. In this context, this work deals with the isolation of hybrid carrageenans from red seaweed using subcritical water extraction operating in a wide range of thermal conditions (70-190 °C). The extracted biopolymers were analyzed by means of either Fourier-Transform infrared, nuclear magnetic resonance, rheological or cell viability assays. In parallel, the fundamental chemical composition of the seaweed used as raw material, as well as the main phytochemical properties of the soluble liquid extracts, were also studied. Results indicated that thermal extraction conditions significantly affected the rheological behavior of the recovered hybrid carrageenans. The hybrid carrageenan extraction yields varied, with results between 10.2 and 30.2% being the highest values obtained at hydrothermal treatment of 130 °C. A wide palette of viscous features was identified for recovered hybrid carrageenans, with the strongest rheology properties observed at the same temperature. It should be remarked that the maximum inhibitory effect was also obtained at 130 °C for both the ovarian carcinoma cell line (A2780) (65%, IC: 0.31 mg/mL) and lung carcinoma cell line (A549) (59%, IC: 0.41 mg/mL).

Citing Articles

-Carrageenan/Chitosan Nanoparticles via Coacervation: Achieving Stability for Tiny Particles.

Klein R, de Almeida D, de Oliveira A, Bonafe E, Monteiro J, Sabino R Nanomaterials (Basel). 2025; 15(3).

PMID: 39940137 PMC: 11819667. DOI: 10.3390/nano15030161.

Piezoelectric Behaviour in Biodegradable Carrageenan and Iron (III) Oxide Based Sensor.

Bucinskas V, Udris D, Dzedzickis A, Petroniene J Sensors (Basel). 2024; 24(14).

PMID: 39066021 PMC: 11280553. DOI: 10.3390/s24144622.

Green Synthesis of Cation Exchange Membranes: A Review.

Depuydt S, Van der Bruggen B Membranes (Basel). 2024; 14(1).

PMID: 38248713 PMC: 10819081. DOI: 10.3390/membranes14010023.

Assessment of Hydrothermal Treatment Effects on Coir Fibers for Incorporation into Polyurethane Matrix Biocomposites Derived from Castor Oil.

Camillo M, Goncalves B, Candido V, Dias L, Moulin J, Monteiro S Polymers (Basel). 2024; 15(23).

PMID: 38232049 PMC: 10708770. DOI: 10.3390/polym15234614.

Subcritical Water Extraction to Valorize Grape Biomass-A Step Closer to Circular Economy.

Ferreira C, Moreira M, Delerue-Matos C, Sarraguca M Molecules. 2023; 28(22).

PMID: 38005259 PMC: 10673199. DOI: 10.3390/molecules28227538.

References

Florez-Fernandez N, Torres M, Gonzalez-Munoz M, Dominguez H . Recovery of bioactive and gelling extracts from edible brown seaweed Laminaria ochroleuca by non-isothermal autohydrolysis. Food Chem. 2018; 277:353-361. DOI: 10.1016/j.foodchem.2018.10.096. View

Gomez-Ordonez E, Alonso E, Ruperez P . A simple ion chromatography method for inorganic anion analysis in edible seaweeds. Talanta. 2010; 82(4):1313-7. DOI: 10.1016/j.talanta.2010.06.062. View

Alves C, Pinteus S, Rodrigues A, Horta A, Pedrosa R . Algae from Portuguese Coast Presented High Cytotoxicity and Antiproliferative Effects on an Model of Human Colorectal Cancer. Pharmacognosy Res. 2018; 10(1):24-30. PMC: 5855369. DOI: 10.4103/pr.pr_151_16. View

Sangroniz L, Fernandez M, Partal P, Santamaria A . Rheology of Polymer Processing in Spain (1995-2020). Polymers (Basel). 2021; 13(14). PMC: 8309276. DOI: 10.3390/polym13142314. View

Balboa E, Rivas S, Moure A, Dominguez H, Parajo J . Simultaneous extraction and depolymerization of fucoidan from Sargassum muticum in aqueous media. Mar Drugs. 2013; 11(11):4612-27. PMC: 3853749. DOI: 10.3390/md11114612. View

Aziz E, Batool R, Khan M, Rauf A, Akhtar W, Heydari M . An overview on red algae bioactive compounds and their pharmaceutical applications. J Complement Integr Med. 2020; . DOI: 10.1515/jcim-2019-0203. View

Yun E, Yu S, Kim Y, Liu J, Kang N, Jin Y . In Vitro Prebiotic and Anti-Colon Cancer Activities of Agar-Derived Sugars from Red Seaweeds. Mar Drugs. 2021; 19(4). PMC: 8070132. DOI: 10.3390/md19040213. View

Afonso C, Correia A, Freitas M, Baptista T, Neves M, Mouga T . Seasonal Changes in the Nutritional Composition of (Rhodophyta, Gracilariales) from the Center of Portugal. Foods. 2021; 10(5). PMC: 8160604. DOI: 10.3390/foods10051145. View

Gonzalez-Ballesteros N, Torres M, Florez-Fernandez N, Diego-Gonzalez L, Simon-Vazquez R, Rodriguez-Arguelles M . Eco-friendly extraction of Mastocarpus stellatus carrageenan for the synthesis of gold nanoparticles with improved biological activity. Int J Biol Macromol. 2021; 183:1436-1449. DOI: 10.1016/j.ijbiomac.2021.05.115. View

10.

Lomartire S, Marques J, Goncalves A . An Overview to the Health Benefits of Seaweeds Consumption. Mar Drugs. 2021; 19(6). PMC: 8232781. DOI: 10.3390/md19060341. View

11.

DODGSON K . Determination of inorganic sulphate in studies on the enzymic and non-enzymic hydrolysis of carbohydrate and other sulphate esters. Biochem J. 1961; 78:312-9. PMC: 1205268. DOI: 10.1042/bj0780312. View

12.

Prasedya E, Miyake M, Kobayashi D, Hazama A . Carrageenan delays cell cycle progression in human cancer cells in vitro demonstrated by FUCCI imaging. BMC Complement Altern Med. 2016; 16:270. PMC: 4973075. DOI: 10.1186/s12906-016-1199-5. View

13.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

14.

Cikos A, Jerkovic I, Molnar M, Subaric D, Jokic S . New trends for macroalgal natural products applications. Nat Prod Res. 2019; 35(7):1180-1191. DOI: 10.1080/14786419.2019.1644629. View

15.

Torres M, Chenlo F, Moreira R . Rheology of κ/ι-hybrid carrageenan from Mastocarpus stellatus: Critical parameters for the gel formation. Int J Biol Macromol. 2016; 86:418-24. DOI: 10.1016/j.ijbiomac.2016.01.096. View

16.

Barral-Martinez M, Florez-Fernandez N, Dominguez H, Torres M . Tailoring hybrid carrageenans from Mastocarpus stellatus red seaweed using microwave hydrodiffusion and gravity. Carbohydr Polym. 2020; 248:116830. DOI: 10.1016/j.carbpol.2020.116830. View

17.

Zainal Ariffin S, Yeen W, Zainol Abidin I, Wahab R, Zainal Ariffin Z, Senafi S . Cytotoxicity effect of degraded and undegraded kappa and iota carrageenan in human intestine and liver cell lines. BMC Complement Altern Med. 2014; 14:508. PMC: 4320596. DOI: 10.1186/1472-6882-14-508. View

18.

Chen X, Zhao X, Gao Y, Yin J, Bai M, Wang F . Green Synthesis of Gold Nanoparticles Using Carrageenan Oligosaccharide and Their In Vitro Antitumor Activity. Mar Drugs. 2018; 16(8). PMC: 6117638. DOI: 10.3390/md16080277. View

19.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M . Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999; 26(9-10):1231-7. DOI: 10.1016/s0891-5849(98)00315-3. View

20.

Koivikko R, Loponen J, Honkanen T, Jormalainen V . Contents of soluble, cell-wall-bound and exuded phlorotannins in the brown alga Fucus vesiculosus, with implications on their ecological functions. J Chem Ecol. 2005; 31(1):195-212. DOI: 10.1007/s10886-005-0984-2. View