Ethanol Promoting the Upregulation of C-X-C Motif Chemokine Ligand 1(CXCL1) and C-X-C Motif Chemokine Ligand 6(CXCL6) in Models of Early Alcoholic Liver Disease
Overview
Affiliations
Alcoholic liver disease (ALD) denotes a series of liver diseases caused by ethanol. Recently, immune-related genes (IRGs) play increasingly crucial role in diseases. However, it's unclear the role of IRGs in ALD. Bioinformatic analysis was used to discern the core immune-related differential genes (IRDGs) in the present study. Subsequently, Cell Counting Kit-8 say, oil red O staining, and triglyceride detection were employed to explore optimal experimental conditions of establishing hepatocellular models of early ALD. Ultimately, real-time reverse transcription-PCR and immunohistochemistry/immunocytochemistry methods were adopted to verify the expressions of mRNA and proteins of core IRDGs, respectively. C-X-C Motif Chemokine Ligand 1 () and were regarded as core IRDGs via integrated bioinformatics analysis. Besides, Lieber Decarli Ethanol feeding and 200 mM and 300 mM ethanol stimulating L02 cells for 36 h can both successfully hepatocellular model. In ethanol groups, the levels of CXCL1 and CXCL6 mRNA were significantly upregulated than pair-fed groups (P < 0.0001). Also, immunohistochemistry revealed that positive particles of CXCL1 and CXCL6 in mice model of early ALD were obviously more than control groups (P < 0.0001). Besides, in L02 hepatocytes stimulated by ethanol, CXCL1 and CXCL6 mRNA were over-expressed, compared with normal L02 cells (P < 0.0001). Meanwhile, immunocytochemistry indicated that CXCL1 and CXCL6 proteins in hepatocellular model of early ALD were higher than normal L02 hepatocytes stimulus (P < 0.0001). Ethanol promoted the upregulation of and mRNA and proteins in models of early ALD, denoting their potentiality of acting as biomarkers of ALD.
Intestinal microbiota homeostasis analysis in riboflavin-treated alcoholic liver disease.
Shen X, Shi C, Xu J, Zhi F, Luo K, Di Y Commun Biol. 2024; 7(1):1030.
PMID: 39169207 PMC: 11339332. DOI: 10.1038/s42003-024-06722-4.
The Clinical Significance and Role of CXCL1 Chemokine in Gastrointestinal Cancers.
Korbecki J, Bosiacki M, Barczak K, Lagocka R, Chlubek D, Baranowska-Bosiacka I Cells. 2023; 12(10).
PMID: 37408240 PMC: 10217339. DOI: 10.3390/cells12101406.
The role of CXCL family members in different diseases.
Zhou C, Gao Y, Ding P, Wu T, Ji G Cell Death Discov. 2023; 9(1):212.
PMID: 37393391 PMC: 10314943. DOI: 10.1038/s41420-023-01524-9.
Myeloid cells in alcoholic liver diseases: Mechanism and prospect.
Xu W, Wu M, Chen B, Wang H Front Immunol. 2022; 13:971346.
PMID: 36032154 PMC: 9399804. DOI: 10.3389/fimmu.2022.971346.