The Spatial Transcriptomic Landscape of the Healing Mouse Intestine Following Damage

Overview

Journal Nat Commun

Specialty Biology

Date 2022 Feb 12

PMID 35149721

Authors

Sara M Parigi

Ludvig Larsson

Srustidhar Das

Ricardo O Ramirez Flores

Annika Frede

Kumar P Tripathi

Oscar E Diaz

Katja Selin

Rodrigo A Morales

Xinxin Luo

Gustavo Monasterio

Camilla Engblom

Nicola Gagliani

Julio Saez-Rodriguez

Joakim Lundeberg

Eduardo J Villablanca

Affiliations

Soon will be listed here.

Abstract

The intestinal barrier is composed of a complex cell network defining highly compartmentalized and specialized structures. Here, we use spatial transcriptomics to define how the transcriptomic landscape is spatially organized in the steady state and healing murine colon. At steady state conditions, we demonstrate a previously unappreciated molecular regionalization of the colon, which dramatically changes during mucosal healing. Here, we identified spatially-organized transcriptional programs defining compartmentalized mucosal healing, and regions with dominant wired pathways. Furthermore, we showed that decreased p53 activation defined areas with increased presence of proliferating epithelial stem cells. Finally, we mapped transcriptomics modules associated with human diseases demonstrating the translational potential of our dataset. Overall, we provide a publicly available resource defining principles of transcriptomic regionalization of the colon during mucosal healing and a framework to develop and progress further hypotheses.

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