RNA MA Alterations Induced by Biomineralization Nanoparticles: A Proof-of-Concept Study of Epitranscriptomics for Nanotoxicity Evaluation

Overview

Journal Nanoscale Res Lett

Publisher Springer

Specialty Biotechnology

Date 2022 Feb 5

PMID 35122526

Authors

Jinbin Pan

Jiaojiao Wang

Kun Fang

Wenjing Hou

Bing Li

Jie Zhao

Xinlong Ma

Affiliations

Soon will be listed here.

Abstract

Although various strategies have been included in nanotoxicity evaluation, epitranscriptomics has rarely been integrated into this field. In this proof-of-concept study, N6-methyladenosine (mA) changes of mRNA in HEK293T cells induced by three bovine serum albumin (BSA)-templated Au, CuS and GdO nanoparticles are systematically explored, and their possible biological mechanisms are preliminarily investigated. It has been found that all the three BSA-templated nanoparticles can reduce mA levels, and the genes with reduced mA are enriched for TGF-beta signaling, which is critical for cell proliferation, differentiation and apoptosis. Further results indicate that abnormal aggregation of mA-related enzymes at least partly account for the nanoparticle-induced epitranscriptomic changes. These findings demonstrate that epitranscriptomics analysis can provide an unprecedented landscape of the biological effect induced by nanomaterials, which should be involved in the nanotoxicity evaluation to promote the potential clinical translation of nanomaterials.

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