Real-time Tracking of a Diffuse Reflectance Spectroscopy Probe Used to Aid Histological Validation of Margin Assessment in Upper Gastrointestinal Cancer Resection Surgery

Overview

Journal J Biomed Opt

Specialties Biomedical Engineering
Ophthalmology

Date 2022 Feb 2

PMID 35106980

Authors

Ioannis Gkouzionis

Scarlet Nazarian

Michal Kawka

Ara Darzi

Nisha Patel

Christopher J Peters

Daniel S Elson

Affiliations

Soon will be listed here.

Abstract

Significance: Diffuse reflectance spectroscopy (DRS) allows discrimination of tissue type. Its application is limited by the inability to mark the scanned tissue and the lack of real-time measurements.

Aim: This study aimed to develop a real-time tracking system to enable localization of a DRS probe to aid the classification of tumor and non-tumor tissue.

Approach: A green-colored marker attached to the DRS probe was detected using hue-saturation-value (HSV) segmentation. A live, augmented view of tracked optical biopsy sites was recorded in real time. Supervised classifiers were evaluated in terms of sensitivity, specificity, and overall accuracy. A developed software was used for data collection, processing, and statistical analysis.

Results: The measured root mean square error (RMSE) of DRS probe tip tracking was 1.18 ± 0.58 mm and 1.05 ± 0.28 mm for the x and y dimensions, respectively. The diagnostic accuracy of the system to classify tumor and non-tumor tissue in real time was 94% for stomach and 96% for the esophagus.

Conclusions: We have successfully developed a real-time tracking and classification system for a DRS probe. When used on stomach and esophageal tissue for tumor detection, the accuracy derived demonstrates the strength and clinical value of the technique to aid margin assessment in cancer resection surgery.

Citing Articles

Detection and characterization of colorectal cancer by autofluorescence lifetime imaging on surgical specimens.

Herrando A, Fernandez L, Azevedo J, Vieira P, Domingos H, Galzerano A Sci Rep. 2024; 14(1):24575.

PMID: 39426971 PMC: 11490491. DOI: 10.1038/s41598-024-74224-8.

Point Projection Mapping System for Tracking, Registering, Labeling, and Validating Optical Tissue Measurements.

Feenstra L, van der Stel S, Da Silva Guimaraes M, Dashtbozorg B, Ruers T J Imaging. 2024; 10(2).

PMID: 38392085 PMC: 10890146. DOI: 10.3390/jimaging10020037.

A YOLOv5-based network for the detection of a diffuse reflectance spectroscopy probe to aid surgical guidance in gastrointestinal cancer surgery.

Gkouzionis I, Zhong Y, Nazarian S, Darzi A, Patel N, Peters C Int J Comput Assist Radiol Surg. 2023; 19(1):11-14.

PMID: 37289279 PMC: 10769906. DOI: 10.1007/s11548-023-02944-9.

Diffuse Reflectance Spectroscopy of the Oral Mucosa: In Vivo Experimental Validation of the Precancerous Lesions Early Detection Possibility.

Kolpakov A, Moshkova A, Melikhova E, Sokolova D, Muravskaya N, Samorodov A Diagnostics (Basel). 2023; 13(9).

PMID: 37175023 PMC: 10177876. DOI: 10.3390/diagnostics13091633.

References

Baltussen E, Sterenborg H, Ruers T, Dashtbozorg B . Optimizing algorithm development for tissue classification in colorectal cancer based on diffuse reflectance spectra. Biomed Opt Express. 2019; 10(12):6096-6113. PMC: 6913395. DOI: 10.1364/BOE.10.006096. View

Evers D, Hendriks B, Lucassen G, Ruers T . Optical spectroscopy: current advances and future applications in cancer diagnostics and therapy. Future Oncol. 2012; 8(3):307-20. DOI: 10.2217/fon.12.15. View

Zhao Z, Chen Z, Voros S, Cheng X . Real-time tracking of surgical instruments based on spatio-temporal context and deep learning. Comput Assist Surg (Abingdon). 2019; 24(sup1):20-29. DOI: 10.1080/24699322.2018.1560097. View

Keller A, Bialecki P, Wilhelm T, Vetter M . Diffuse reflectance spectroscopy of human liver tumor specimens - towards a tissue differentiating optical biopsy needle using light emitting diodes. Biomed Opt Express. 2018; 9(3):1069-1081. PMC: 5846514. DOI: 10.1364/BOE.9.001069. View

Bouarfa L, Akman O, Schneider A, Jonker P, Dankelman J . In-vivo real-time tracking of surgical instruments in endoscopic video. Minim Invasive Ther Allied Technol. 2011; 21(3):129-34. DOI: 10.3109/13645706.2011.580764. View

Mountney P, Giannarou S, Elson D, Yang G . Optical biopsy mapping for minimally invasive cancer screening. Med Image Comput Comput Assist Interv. 2010; 12(Pt 1):483-90. DOI: 10.1007/978-3-642-04268-3_60. View

Nachabe R, Evers D, Hendriks B, Lucassen G, van der Voort M, Rutgers E . Diagnosis of breast cancer using diffuse optical spectroscopy from 500 to 1600 nm: comparison of classification methods. J Biomed Opt. 2011; 16(8):087010. DOI: 10.1117/1.3611010. View

Biondi A, Persiani R, Cananzi F, Zoccali M, Vigorita V, Tufo A . R0 resection in the treatment of gastric cancer: room for improvement. World J Gastroenterol. 2010; 16(27):3358-70. PMC: 2904881. DOI: 10.3748/wjg.v16.i27.3358. View

Balog J, Sasi-Szabo L, Kinross J, Lewis M, Muirhead L, Veselkov K . Intraoperative tissue identification using rapid evaporative ionization mass spectrometry. Sci Transl Med. 2013; 5(194):194ra93. DOI: 10.1126/scitranslmed.3005623. View

10.

Bergholt M, Zheng W, Ho K, Teh M, Yeoh K, So J . Fiber-optic Raman spectroscopy probes gastric carcinogenesis in vivo at endoscopy. J Biophotonics. 2013; 6(1):49-59. DOI: 10.1002/jbio.201200138. View

11.

St John E, Balog J, McKenzie J, Rossi M, Covington A, Muirhead L . Rapid evaporative ionisation mass spectrometry of electrosurgical vapours for the identification of breast pathology: towards an intelligent knife for breast cancer surgery. Breast Cancer Res. 2017; 19(1):59. PMC: 5442854. DOI: 10.1186/s13058-017-0845-2. View

12.

Elfring R, de la Fuente M, Radermacher K . Assessment of optical localizer accuracy for computer aided surgery systems. Comput Aided Surg. 2010; 15(1-3):1-12. DOI: 10.3109/10929081003647239. View

13.

Ni Z, Bian G, Xie X, Hou Z, Zhou X, Zhou Y . RASNet: Segmentation for Tracking Surgical Instruments in Surgical Videos Using Refined Attention Segmentation Network. Annu Int Conf IEEE Eng Med Biol Soc. 2020; 2019:5735-5738. DOI: 10.1109/EMBC.2019.8856495. View

14.

Huang B, Tsai Y, Cartucho J, Vyas K, Tuch D, Giannarou S . Tracking and visualization of the sensing area for a tethered laparoscopic gamma probe. Int J Comput Assist Radiol Surg. 2020; 15(8):1389-1397. PMC: 7351835. DOI: 10.1007/s11548-020-02205-z. View

15.

Dexter S, Sue-Ling H, McMahon M, Quirke P, Mapstone N, Martin I . Circumferential resection margin involvement: an independent predictor of survival following surgery for oesophageal cancer. Gut. 2001; 48(5):667-70. PMC: 1728263. DOI: 10.1136/gut.48.5.667. View

16.

Baltussen E, Snaebjornsson P, de Koning S, Sterenborg H, Aalbers A, Kok N . Diffuse reflectance spectroscopy as a tool for real-time tissue assessment during colorectal cancer surgery. J Biomed Opt. 2017; 22(10):1-6. DOI: 10.1117/1.JBO.22.10.106014. View

17.

Bouget D, Allan M, Stoyanov D, Jannin P . Vision-based and marker-less surgical tool detection and tracking: a review of the literature. Med Image Anal. 2016; 35:633-654. DOI: 10.1016/j.media.2016.09.003. View

18.

Zysk A, Chen K, Gabrielson E, Tafra L, May Gonzalez E, Canner J . Intraoperative Assessment of Final Margins with a Handheld Optical Imaging Probe During Breast-Conserving Surgery May Reduce the Reoperation Rate: Results of a Multicenter Study. Ann Surg Oncol. 2015; 22(10):3356-62. PMC: 4839389. DOI: 10.1245/s10434-015-4665-2. View

19.

Juez L, Barranquero A, Priego P, Cuadrado M, Blazquez L, Sanchez-Picot S . Influence of positive margins on tumour recurrence and overall survival after gastrectomy for gastric cancer. ANZ J Surg. 2021; 91(7-8):E465-E473. DOI: 10.1111/ans.16937. View

20.

Du X, Kurmann T, Chang P, Allan M, Ourselin S, Sznitman R . Articulated Multi-Instrument 2-D Pose Estimation Using Fully Convolutional Networks. IEEE Trans Med Imaging. 2018; 37(5):1276-1287. PMC: 6051486. DOI: 10.1109/TMI.2017.2787672. View