Red Light Regulates the Metabolite Biosynthesis in the Leaves of "Huangjinya" Through Amino Acid and Phenylpropanoid Metabolisms

Overview

Journal Front Plant Sci

Date 2022 Jan 31

PMID 35095983

Authors

Qingping Ma

Laichao Song

Zhanhai Niu

Jingshan Li

Yu Wang

Haiwei Sun

Zhihong Ren

Hongxia Zhao

Shangjing Guo

Zhaotang Ding

Affiliations

Soon will be listed here.

Abstract

"Huangjinya" is a light-sensitive albino variety and is widely cultivated in China. It has been proved that red light could promote the vegetable growth of plants. However, the mechanism of "Huangjinya" in response to a red light is unclear. This study used high-throughput sequencing technology to analyze the transcriptome of tender shoots of "Huangjinya" under the white and red light supplement conditions. At the same time, liquid chromatography tandem mass spectrometry (LC-MS) was used to analyze metabolite changes under different light conditions. Transcriptome analysis revealed that a total of 174 differentially expressed genes (DEGs) were identified after the red light supplement. Kyoto encyclopedia of genes and genomes (KEGG) classification indicated that amino acid metabolism enriched the most DEGs. In addition, two phenylpropanoid metabolism-related genes and five glutathione -transferase genes () were found to be expressed differently. Metabolome analysis revealed that 193 differential metabolites were obtained. Being the same as transcriptome analysis, most differential metabolites were enriched in amino acids, sweet and umami tasting amino acids were increased, and bitter-tasting amino acids were decreased after the red light supplement. In summary, red light supplementary treatment may be propitious to the quality of "Huangjinya" due to its regulatory effect on amino acid metabolism. Also, involved phenylpropanoid metabolism contributed to tea quality changes in "Huangjinya."

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References

Xia E, Tong W, Hou Y, An Y, Chen L, Wu Q . The Reference Genome of Tea Plant and Resequencing of 81 Diverse Accessions Provide Insights into Its Genome Evolution and Adaptation. Mol Plant. 2020; 13(7):1013-1026. DOI: 10.1016/j.molp.2020.04.010. View

Zhang Q, Liu M, Ruan J . Integrated Transcriptome and Metabolic Analyses Reveals Novel Insights into Free Amino Acid Metabolism in Tea Cultivar. Front Plant Sci. 2017; 8:291. PMC: 5337497. DOI: 10.3389/fpls.2017.00291. View

Hu B, Zhao J, Lai B, Qin Y, Wang H, Hu G . LcGST4 is an anthocyanin-related glutathione S-transferase gene in Litchi chinensis Sonn. Plant Cell Rep. 2016; 35(4):831-43. DOI: 10.1007/s00299-015-1924-4. View

Kou M, Liu Y, Li Z, Zhang Y, Tang W, Yan H . A novel glutathione S-transferase gene from sweetpotato, IbGSTF4, is involved in anthocyanin sequestration. Plant Physiol Biochem. 2019; 135:395-403. DOI: 10.1016/j.plaphy.2018.12.028. View

Cao K, Yu J, Xu D, Ai K, Bao E, Zou Z . Exposure to lower red to far-red light ratios improve tomato tolerance to salt stress. BMC Plant Biol. 2018; 18(1):92. PMC: 5968587. DOI: 10.1186/s12870-018-1310-9. View

Grefen C, Harter K . Plant two-component systems: principles, functions, complexity and cross talk. Planta. 2004; 219(5):733-42. DOI: 10.1007/s00425-004-1316-4. View

Luo H, Dai C, Li Y, Feng J, Liu Z, Kang C . Reduced Anthocyanins in Petioles codes for a GST anthocyanin transporter that is essential for the foliage and fruit coloration in strawberry. J Exp Bot. 2018; 69(10):2595-2608. PMC: 5920330. DOI: 10.1093/jxb/ery096. View

Holalu S, Finlayson S . The ratio of red light to far red light alters Arabidopsis axillary bud growth and abscisic acid signalling before stem auxin changes. J Exp Bot. 2017; 68(5):943-952. PMC: 5444464. DOI: 10.1093/jxb/erw479. View

Bachmanov A, Bosak N, Glendinning J, Inoue M, Li X, Manita S . Genetics of Amino Acid Taste and Appetite. Adv Nutr. 2016; 7(4):806S-22S. PMC: 4942865. DOI: 10.3945/an.115.011270. View

10.

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

11.

Feng L, Gao M, Hou R, Hu X, Zhang L, Wan X . Determination of quality constituents in the young leaves of albino tea cultivars. Food Chem. 2014; 155:98-104. DOI: 10.1016/j.foodchem.2014.01.044. View

12.

Oh J, Park E, Song K, Bae G, Choi G . PHYTOCHROME INTERACTING FACTOR8 Inhibits Phytochrome A-Mediated Far-Red Light Responses in Arabidopsis. Plant Cell. 2019; 32(1):186-205. PMC: 6961613. DOI: 10.1105/tpc.19.00515. View

13.

Kumar S, Stecher G, Tamura K . MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol Biol Evol. 2016; 33(7):1870-4. PMC: 8210823. DOI: 10.1093/molbev/msw054. View

14.

Pedmale U, Huang S, Zander M, Cole B, Hetzel J, Ljung K . Cryptochromes Interact Directly with PIFs to Control Plant Growth in Limiting Blue Light. Cell. 2016; 164(1-2):233-245. PMC: 4721562. DOI: 10.1016/j.cell.2015.12.018. View

15.

Dong F, Shi Y, Liu M, Fan K, Zhang Q, Ruan J . iTRAQ-Based Quantitative Proteomics Analysis Reveals the Mechanism Underlying the Weakening of Carbon Metabolism in Chlorotic Tea Leaves. Int J Mol Sci. 2018; 19(12). PMC: 6321456. DOI: 10.3390/ijms19123943. View

16.

Song L, Ma Q, Zou Z, Sun K, Yao Y, Tao J . Molecular Link between Leaf Coloration and Gene Expression of Flavonoid and Carotenoid Biosynthesis in Cultivar 'Huangjinya'. Front Plant Sci. 2017; 8:803. PMC: 5443146. DOI: 10.3389/fpls.2017.00803. View

17.

Bakuradze T, Tausend A, Galan J, Groh I, Berry D, Tur J . Antioxidative activity and health benefits of anthocyanin-rich fruit juice in healthy volunteers. Free Radic Res. 2019; 53(sup1):1045-1055. DOI: 10.1080/10715762.2019.1618851. View

18.

Ballare C . Light regulation of plant defense. Annu Rev Plant Biol. 2014; 65:335-63. DOI: 10.1146/annurev-arplant-050213-040145. View

19.

Yu Y, Kou X, Gao R, Chen X, Zhao Z, Mei H . Glutamine Synthetases Play a Vital Role in High Accumulation of Theanine in Tender Shoots of Albino Tea Germplasm "Huabai 1". J Agric Food Chem. 2021; 69(46):13904-13915. DOI: 10.1021/acs.jafc.1c04567. View

20.

de Wit M, Keuskamp D, Bongers F, Hornitschek P, Gommers C, Reinen E . Integration of Phytochrome and Cryptochrome Signals Determines Plant Growth during Competition for Light. Curr Biol. 2016; 26(24):3320-3326. DOI: 10.1016/j.cub.2016.10.031. View