Arcopilus Eremanthusum Sp. Nov. As Sources of Antibacterial and Antioxidant Metabolites

Overview

Journal Arch Microbiol

Specialty Microbiology

Date 2022 Jan 30

PMID 35094184

Authors

Derica Goncalves Tavares

Sarah da Silva Costa Guimaraes

Roberta Hilsdorf Piccoli

Whasley Ferreira Duarte

Patricia Gomes Cardoso

Affiliations

Soon will be listed here.

Abstract

In this study, we evaluated the antibacterial and antioxidant activities of ethyl acetate (EtOAc) extracts of Arcopilus eremanthusum sp. nov. (CML3766) isolated from E. erythopappus. The fungi were identified using the sequences of the internal transcribed spacer (ITS), large subunit (LSU), and RNA polymerase II second largest subunit (RPB2). Antibacterial activity was determined using the minimum bactericidal concentration (MBC) method. Antioxidant activity was evaluated using free radical scavenging methods with 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assays and the β-carotene-linoleic acid system. The total phenolic compound content was measured using the Folin-Ciocalteu method. The endophytic fungal extract presented bactericidal activity, with an MBC of 2.44 and 19.5 μg/mL against Staphylococcus aureus GL 8702 and GL 5674, respectively, and 625 μg/mL for Salmonella enterica serovar Enteritidis. In addition, this fungus demonstrated an antioxidant activity of 52.30% protection in the β-carotene method. The total concentration of phenolic compounds was 23.73 mg gallic acid equivalent (GAE)/g. Ferulic acid, trans-cinnamic acid, chlorogenic acid, catechin, vanillin, p-coumaric acid, and caffeic acid were quantified using high-performance liquid chromatography with diode array detection. The results demonstrate the potential of A. eremanthusum sp. nov. to serve as a source of antibacterial and antioxidant metabolites with possible future biotechnological applications.

Citing Articles

A Review on Electrochemical Sensors and Biosensors Used in Assessing Antioxidant Activity.

Munteanu I, Apetrei C Antioxidants (Basel). 2022; 11(3).

PMID: 35326234 PMC: 8945540. DOI: 10.3390/antiox11030584.

References

Borges C, Minatel I, Amorim E, Belin M, Gomez-Gomez H, Correa C . Ripening and cooking processes influence the carotenoid content in bananas and plantains (Musa spp.). Food Res Int. 2019; 124:129-136. DOI: 10.1016/j.foodres.2018.08.022. View

Awad N, Kassem H, Hamed M, El-Naggar M, El-Feky A . Bioassays guided isolation of compounds from Chaetomium globosum. J Mycol Med. 2013; 24(2):e35-42. DOI: 10.1016/j.mycmed.2013.10.005. View

Brewer D, JEN W, Jones G, Taylor A . The antibacterial activity of some naturally occurring 2,5-dihydroxy-1,4-benzoquinones. Can J Microbiol. 1984; 30(8):1068-72. DOI: 10.1139/m84-166. View

Dwibedi V, Saxena S . Arcopilus aureus, a Resveratrol-Producing Endophyte from Vitis vinifera. Appl Biochem Biotechnol. 2018; 186(2):476-495. DOI: 10.1007/s12010-018-2755-x. View

Ekmekcioglu C, Feyertag J, Marktl W . Cinnamic acid inhibits proliferation and modulates brush border membrane enzyme activities in Caco-2 cells. Cancer Lett. 1998; 128(2):137-44. DOI: 10.1016/s0304-3835(98)00073-1. View

Graf E . Antioxidant potential of ferulic acid. Free Radic Biol Med. 1992; 13(4):435-48. DOI: 10.1016/0891-5849(92)90184-i. View

Hamad B . The antibiotics market. Nat Rev Drug Discov. 2010; 9(9):675-6. DOI: 10.1038/nrd3267. View

Hampton T . Novel Programs and Discoveries Aim to Combat Antibiotic Resistance. JAMA. 2015; 313(24):2411-3. DOI: 10.1001/jama.2015.4738. View

Hirabayashi T, Ochiai H, Sakai S, Nakajima K, Terasawa K . Inhibitory effect of ferulic acid and isoferulic acid on murine interleukin-8 production in response to influenza virus infections in vitro and in vivo. Planta Med. 1995; 61(3):221-6. DOI: 10.1055/s-2006-958060. View

10.

Kabbaj F, Lu S, Faouzi M, Meddah B, Proksch P, Cherrah Y . Bioactive metabolites from Chaetomium aureum: structure elucidation and inhibition of the Hsp90 machine chaperoning activity. Bioorg Med Chem. 2014; 23(1):126-31. PMC: 4274242. DOI: 10.1016/j.bmc.2014.11.021. View

11.

Kamel N, Abdel-Motaal F, El-Zayat S . Endophytic fungi from the medicinal herb Euphorbia geniculata as a potential source for bioactive metabolites. Arch Microbiol. 2019; 202(2):247-255. DOI: 10.1007/s00203-019-01740-x. View

12.

Kanokmedhakul S, Kanokmedhakul K, Nasomjai P, Louangsysouphanh S, Soytong K, Isobe M . Antifungal azaphilones from the fungus Chaetomium cupreum CC3003. J Nat Prod. 2006; 69(6):891-5. DOI: 10.1021/np060051v. View

13.

OSEID S . [Exercise in thin air]. Tidsskr Nor Laegeforen. 1966; 86(14):1066-8. View

14.

Lee S, Park H, Nguyen T, Lee H . Characterization of Three Species of Sordariomycetes Isolated from Freshwater and Soil Samples in Korea. Mycobiology. 2019; 47(1):20-30. PMC: 6450575. DOI: 10.1080/12298093.2019.1574372. View

15.

Mathew S, Abraham T . Ferulic acid: an antioxidant found naturally in plant cell walls and feruloyl esterases involved in its release and their applications. Crit Rev Biotechnol. 2004; 24(2-3):59-83. DOI: 10.1080/07388550490491467. View

16.

Oyinloye B, Adenowo A, Kappo A . Reactive oxygen species, apoptosis, antimicrobial peptides and human inflammatory diseases. Pharmaceuticals (Basel). 2015; 8(2):151-75. PMC: 4491653. DOI: 10.3390/ph8020151. View

17.

Rababah T, Ereifej K, Howard L . Effect of ascorbic acid and dehydration on concentrations of total phenolics, antioxidant capacity, anthocyanins, and color in fruits. J Agric Food Chem. 2005; 53(11):4444-7. DOI: 10.1021/jf0502810. View

18.

Rice-Evans C, Miller N, Paganga G . Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med. 1996; 20(7):933-56. DOI: 10.1016/0891-5849(95)02227-9. View

19.

Sato Y, Itagaki S, Kurokawa T, Ogura J, Kobayashi M, Hirano T . In vitro and in vivo antioxidant properties of chlorogenic acid and caffeic acid. Int J Pharm. 2010; 403(1-2):136-8. DOI: 10.1016/j.ijpharm.2010.09.035. View

20.

Torres Y Torres J, Rosazza J . Microbial transformations of p-coumaric acid by Bacillus megaterium and Curvularia lunata. J Nat Prod. 2001; 64(11):1408-14. DOI: 10.1021/np010238g. View