The Hepatocyte Primary Culture/DNA Repair Test Using Hepatocytes from Several Species

Overview

Journal Cell Biol Toxicol

Publisher Springer

Specialties Cell Biology
Toxicology

Date 1987 Jun 1

PMID 3507256

Citations 3

Authors

C A McQueen

G M Williams

Affiliations

Soon will be listed here.

Abstract

The hepatocyte primary culture/DNA repair test, originally validated with rat hepatocytes, has been extended to use hepatocytes from other species including mouse, hamster, guinea pig, rabbit, monkey and human. Both qualitative and quantitative differences have been observed when chemicals are examined in the hepatocyte primary culture/DNA repair test using hepatocytes from more than one species. Examples are discussed that illustrate that the genotoxicity of a chemical can be a species-specific response and that multi-species testing permits a more complete assessment of genotoxicity.

Citing Articles

Mechanisms of DNA-reactive and epigenetic chemical carcinogens: applications to carcinogenicity testing and risk assessment.

Kobets T, Iatropoulos M, Williams G Toxicol Res (Camb). 2019; 8(2):123-145.

PMID: 30997017 PMC: 6417487. DOI: 10.1039/c8tx00250a.

Dehydropyrrolizidine Alkaloid Toxicity, Cytotoxicity, and Carcinogenicity.

Stegelmeier B, Colegate S, Brown A Toxins (Basel). 2016; 8(12).

PMID: 27916846 PMC: 5198550. DOI: 10.3390/toxins8120356.

Protein interferon-stimulated gene 15 conjugation delays but does not overcome coronavirus proliferation in a model of fulminant hepatitis.

Ma X, Bartczak A, Zhang J, He W, Shalev I, Smil D J Virol. 2014; 88(11):6195-204.

PMID: 24648452 PMC: 4093886. DOI: 10.1128/JVI.03801-13.

References

Butterworth B, Earle L, Strom S, Jirtle R, Michalopoulos G . Induction of DNA repair in human and rat hepatocytes by 1,6-dinitropyrene. Mutat Res. 1983; 122(1):73-80. DOI: 10.1016/0165-7992(83)90145-8. View

Miller E, Miller J, Enomoto M . THE COMPARATIVE CARCINOGENICITIES OF 2-ACETYLAMINOFLUORENE AND ITS N-HYDROXY METABOLITE IN MICE, HAMSTERS, AND GUINEA PIGS. Cancer Res. 1964; 24:2018-31. View

McQueen C, Kreiser D, Williams G . The hepatocyte primary culture/DNA repair assay using mouse or hamster hepatocytes. Environ Mutagen. 1983; 5(1):1-8. DOI: 10.1002/em.2860050102. View

Williams G, Laspia M, Mori H, Hirono I . Genotoxicity of cycasin in the hepatocyte primary culture/DNA repair test supplemented with beta-glucosidase. Cancer Lett. 1981; 12(4):329-33. DOI: 10.1016/0304-3835(81)90175-0. View

Mori H, Kawai K, Ohbayashi F, Kuniyasu T, Yamazaki M, Hamasaki T . Genotoxicity of a variety of mycotoxins in the hepatocyte primary culture/DNA repair test using rat and mouse hepatocytes. Cancer Res. 1984; 44(7):2918-23. View

McQueen C, Williams G . The use of cells from rat, mouse, hamster, and rabbit in the hepatocyte primary culture/DNA-repair test. Ann N Y Acad Sci. 1983; 407:119-30. DOI: 10.1111/j.1749-6632.1983.tb47818.x. View

Williams G, Mazue G, McQueen C, Shimada T . Genotoxicity of the antihypertensive drugs hydralazine and dihydralazine. Science. 1980; 210(4467):329-30. DOI: 10.1126/science.7423193. View

Williams G . Detection of chemical carcinogens by unscheduled DNA synthesis in rat liver primary cell cultures. Cancer Res. 1977; 37(6):1845-51. View

Kornbrust D, Barfknecht T . Comparison of 7 azo dyes and their azo reduction products in the rat and hamster hepatocyte primary culture/DNA-repair assays. Mutat Res. 1984; 136(3):255-66. DOI: 10.1016/0165-1218(84)90059-4. View

10.

. Long-term and short-term screening assays for carcinogens: a critical appraisal. IARC Monogr Eval Carcinog Risk Chem Hum Suppl. 1980; (2 Suppl):1-426. View

11.

Williams G, Gunn J . Long-term cell culture of adult rat liver epithelial cells. Exp Cell Res. 1974; 89(1):139-42. DOI: 10.1016/0014-4827(74)90196-7. View

12.

Reese J, Byard J . Isolation and culture of adult hepatocytes from liver biopsies. In Vitro. 1981; 17(11):935-40. DOI: 10.1007/BF02618417. View

13.

Frymoyer J, Jacox R . INVESTIGATION OF THE GENETIC CONTROL OF SULFADIAZINE AND ISONIAZID METABOLISM IN THE RABBIT. J Lab Clin Med. 1963; 62:891-904. View

14.

Mirsalis J, Butterworth B . Detection of unscheduled DNA synthesis in hepatocytes isolated from rats treated with genotoxic agents: an in vivo- in vitro assay for potential carcinogens and mutagens. Carcinogenesis. 1980; 1(7):621-5. DOI: 10.1093/carcin/1.7.621. View

15.

Williams G, Laspia M . The detection of various nitrosamines in the hepatocyte primary culture/DNA repair test. Cancer Lett. 1979; 6(4-5):199-206. DOI: 10.1016/s0304-3835(79)80034-8. View

16.

Kornbrust D, Barfknecht T . Comparison of rat and hamster hepatocyte primary culture/DNA repair assays. Environ Mutagen. 1984; 6(1):1-11. DOI: 10.1002/em.2860060102. View

17.

Shimada T, Swanson A, Leber P, Williams G . Activities of chlorinated ethane and ethylene compounds in the Salmonella/rat microsome mutagenesis and rat hepatocyte/DNA repair assays under vapor phase exposure conditions. Cell Biol Toxicol. 1985; 1(3):159-79. DOI: 10.1007/BF00120162. View

18.

Strom S, Novicki D, Novotny A, Jirtle R, Michalopoulos G . Human hepatocyte-mediated mutagenesis and DNA repair activity. Carcinogenesis. 1983; 4(6):683-6. DOI: 10.1093/carcin/4.6.683. View

19.

Gordon G, Shafizadeh A, Peters J . Polymorphic acetylation of drugs in rabbits. Xenobiotica. 1973; 3(3):133-50. DOI: 10.3109/00498257309151508. View

20.

McQueen C, Maslansky C, Crescenzi S, Williams G . The genotoxicity of 4,4'-methylenebis-2-chloroaniline in rat, mouse, and hamster hepatocytes. Toxicol Appl Pharmacol. 1981; 58(2):231-5. DOI: 10.1016/0041-008x(81)90427-0. View