Fluorescence Microscopy-An Outline of Hardware, Biological Handling, and Fluorophore Considerations

Overview

Journal Cells

Publisher MDPI

Specialties Biochemistry
Biophysics
Cell Biology
Molecular Biology

Date 2022 Jan 11

PMID 35011596

Authors

Shane M Hickey

Ben Ung

Christie Bader

Robert Brooks

Joanna Lazniewska

Ian R D Johnson

Alexandra Sorvina

Jessica Logan

Carmela Martini

Courtney R Moore

Litsa Karageorgos

Martin J Sweetman

Douglas A Brooks

Affiliations

Soon will be listed here.

Abstract

Fluorescence microscopy has become a critical tool for researchers to understand biological processes at the cellular level. Micrographs from fixed and live-cell imaging procedures feature in a plethora of scientific articles for the field of cell biology, but the complexities of fluorescence microscopy as an imaging tool can sometimes be overlooked or misunderstood. This review seeks to cover the three fundamental considerations when designing fluorescence microscopy experiments: (1) hardware availability; (2) amenability of biological models to fluorescence microscopy; and (3) suitability of imaging agents for intended applications. This review will help equip the reader to make judicious decisions when designing fluorescence microscopy experiments that deliver high-resolution and informative images for cell biology.

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