Determination of Floral Origin Markers of Latvian Honey by Using IRMS, UHPLC-HRMS, and H-NMR

Overview

Journal Foods

Specialty Biotechnology

Date 2022 Jan 11

PMID 35010167

Authors

Kriss Davids Labsvards

Vita Rudovica

Rihards Kluga

Janis Rusko

Lauma Busa

Maris Bertins

Ineta Eglite

Jevgenija Naumenko

Marina Salajeva

Arturs Viksna

Affiliations

Soon will be listed here.

Abstract

The economic significance of honey production is crucial; therefore, modern and efficient methods of authentication are needed. During the last decade, various data processing methods and a combination of several instrumental methods have been increasingly used in food analysis. In this study, the chemical composition of monofloral buckwheat (), clover (), heather (), linden (), rapeseed (), willow (), and polyfloral honey samples of Latvian origin were investigated using several instrumental analysis methods. The data from light stable isotope ratio mass spectrometry (IRMS), ultra-high performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-HRMS), and nuclear magnetic resonance (NMR) analysis methods were used in combination with multivariate analysis to characterize honey samples originating from Latvia. Results were processed using the principal component analysis (PCA) to study the potential possibilities of evaluating the differences between honey of different floral origins. The results indicate the possibility of strong differentiation of heather and buckwheat honeys, and minor differentiation of linden honey from polyfloral honey types. The main indicators include depleted δN values for heather honey protein, elevated concentration levels of rutin for buckwheat honey, and qualitative presence of specific biomarkers within NMR for linden honey.

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