Reactive Oxygen Species-Mediated Cytotoxicity in Liver Carcinoma Cells Induced by Silver Nanoparticles Biosynthesized Using   Extract

Overview

Journal Nanomaterials (Basel)

Date 2022 Jan 11

PMID 35010111

Authors

Waleed Ali Hailan

Khalid Mashay Al-Anazi

Mohammad Abul Farah

Mohammad Ajmal Ali

Ahmed Ali Al-Kawmani

Faisal Mohammed Abou-Tarboush

Affiliations

Soon will be listed here.

Abstract

Hepatocellular carcinoma (HCC) is the most common primary liver malignancy and is ranked as the third most common cause of cancer-related mortality worldwide. () L. is an important medicinal plant that contains many bioactive compounds with pharmacological properties. The role of leaf extract in the biosynthesis of silver nanoparticles (AgNPs) was determined. The biosynthesized AgNPs were thoroughly characterized by UV-vis spectrophotometry, transmission electron microscopy (TEM), X-ray diffraction (XRD), and dynamic light scattering (DLS) techniques. Furthermore, the cytotoxic effect of the biosynthesized AgNPs using (SMAgNPs) against HepG2 liver cancer cells was investigated. Reactive oxygen species generation, apoptosis induction, DNA damage, and autophagy activity were analyzed. The results clearly showed that the biosynthesized silver nanoparticles inhibited the proliferation of HepG2 by significantly ( < 0.05) inducing oxidative stress, cytotoxicity, DNA damage, apoptosis, and autophagy in a dose- and time-dependent manner. These findings may encourage integrating the potential of natural products and the efficiency of silver nanoparticles for the fabrication of safe, environmentally friendly, and effective anticancer agents.

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References

Li Y, Qin T, Ingle T, Yan J, He W, Yin J . Differential genotoxicity mechanisms of silver nanoparticles and silver ions. Arch Toxicol. 2016; 91(1):509-519. DOI: 10.1007/s00204-016-1730-y. View

Abdel-Sattar E, Zaitoun A, Farag M, El Gayed S, Harraz F . Chemical composition, insecticidal and insect repellent activity of Schinus molle L. leaf and fruit essential oils against Trogoderma granarium and Tribolium castaneum. Nat Prod Res. 2009; 24(3):226-35. DOI: 10.1080/14786410802346223. View

Piao M, Kang K, Lee I, Kim H, Kim S, Choi J . Silver nanoparticles induce oxidative cell damage in human liver cells through inhibition of reduced glutathione and induction of mitochondria-involved apoptosis. Toxicol Lett. 2010; 201(1):92-100. DOI: 10.1016/j.toxlet.2010.12.010. View

Ahmadian E, Dizaj S, Rahimpour E, Hasanzadeh A, Eftekhari A, Hosain Zadegan H . Effect of silver nanoparticles in the induction of apoptosis on human hepatocellular carcinoma (HepG2) cell line. Mater Sci Eng C Mater Biol Appl. 2018; 93:465-471. DOI: 10.1016/j.msec.2018.08.027. View

de Mendonca Rocha P, Rodilla J, Diez D, Elder H, Guala M, Silva L . Synergistic antibacterial activity of the essential oil of aguaribay (Schinus molle L.). Molecules. 2012; 17(10):12023-36. PMC: 6268320. DOI: 10.3390/molecules171012023. View

Elmore S . Apoptosis: a review of programmed cell death. Toxicol Pathol. 2007; 35(4):495-516. PMC: 2117903. DOI: 10.1080/01926230701320337. View

Asharani P, Mun G, Hande M, Valiyaveettil S . Cytotoxicity and genotoxicity of silver nanoparticles in human cells. ACS Nano. 2009; 3(2):279-90. DOI: 10.1021/nn800596w. View

Jemal A, Bray F, Center M, Ferlay J, Ward E, Forman D . Global cancer statistics. CA Cancer J Clin. 2011; 61(2):69-90. DOI: 10.3322/caac.20107. View

Bin-Jumah M, Al-Abdan M, Albasher G, Alarifi S . Effects of Green Silver Nanoparticles on Apoptosis and Oxidative Stress in Normal and Cancerous Human Hepatic Cells in vitro. Int J Nanomedicine. 2020; 15:1537-1548. PMC: 7074819. DOI: 10.2147/IJN.S239861. View

10.

Farah M, Ali M, Chen S, Li Y, Al-Hemaid F, Abou-Tarboush F . Silver nanoparticles synthesized from Adenium obesum leaf extract induced DNA damage, apoptosis and autophagy via generation of reactive oxygen species. Colloids Surf B Biointerfaces. 2016; 141:158-169. DOI: 10.1016/j.colsurfb.2016.01.027. View

11.

Kim D, Yang J, Maiti K, Hwang J, Kim K, Seen D . A gonadotropin-releasing hormone-II antagonist induces autophagy of prostate cancer cells. Cancer Res. 2009; 69(3):923-31. DOI: 10.1158/0008-5472.CAN-08-2115. View

12.

Burd J, Usategui-Gomez M . A colorimetric assay for serum lactate dehydrogenase. Clin Chim Acta. 1973; 46(3):223-7. DOI: 10.1016/0009-8981(73)90174-5. View

13.

Baskic D, Popovic S, Ristic P, Arsenijevic N . Analysis of cycloheximide-induced apoptosis in human leukocytes: fluorescence microscopy using annexin V/propidium iodide versus acridin orange/ethidium bromide. Cell Biol Int. 2006; 30(11):924-32. DOI: 10.1016/j.cellbi.2006.06.016. View

14.

Foldbjerg R, Olesen P, Hougaard M, Dang D, Hoffmann H, Autrup H . PVP-coated silver nanoparticles and silver ions induce reactive oxygen species, apoptosis and necrosis in THP-1 monocytes. Toxicol Lett. 2009; 190(2):156-62. DOI: 10.1016/j.toxlet.2009.07.009. View

15.

Hamida R, Albasher G, Bin-Meferij M . Oxidative Stress and Apoptotic Responses Elicited by -Synthesized Silver Nanoparticles against Different Cancer Cell Lines. Cancers (Basel). 2020; 12(8). PMC: 7464693. DOI: 10.3390/cancers12082099. View

16.

Vasanth K, Ilango K, Mohankumar R, Agrawal A, Dubey G . Anticancer activity of Moringa oleifera mediated silver nanoparticles on human cervical carcinoma cells by apoptosis induction. Colloids Surf B Biointerfaces. 2014; 117:354-9. DOI: 10.1016/j.colsurfb.2014.02.052. View

17.

Ratan Z, Haidere M, Nurunnabi M, Shahriar S, Saleh Ahammad A, Shim Y . Green Chemistry Synthesis of Silver Nanoparticles and Their Potential Anticancer Effects. Cancers (Basel). 2020; 12(4). PMC: 7226404. DOI: 10.3390/cancers12040855. View

18.

Su J, Lai H, Chen J, Li L, Wong Y, Chen T . Natural borneol, a monoterpenoid compound, potentiates selenocystine-induced apoptosis in human hepatocellular carcinoma cells by enhancement of cellular uptake and activation of ROS-mediated DNA damage. PLoS One. 2013; 8(5):e63502. PMC: 3658975. DOI: 10.1371/journal.pone.0063502. View

19.

Wilde L, Tanson K, Curry J, Martinez-Outschoorn U . Autophagy in cancer: a complex relationship. Biochem J. 2018; 475(11):1939-1954. DOI: 10.1042/BCJ20170847. View

20.

Ahmed S, Ahmad M, Swami B, Ikram S . A review on plants extract mediated synthesis of silver nanoparticles for antimicrobial applications: A green expertise. J Adv Res. 2016; 7(1):17-28. PMC: 4703479. DOI: 10.1016/j.jare.2015.02.007. View

Reactive Oxygen Species-Mediated Cytotoxicity in Liver Carcinoma Cells Induced by Silver Nanoparticles Biosynthesized Using &nbsp; Extract

Reactive Oxygen Species-Mediated Cytotoxicity in Liver Carcinoma Cells Induced by Silver Nanoparticles Biosynthesized Using Extract