Cytotoxicity Effect of Iron Oxide (FeO)/Graphene Oxide (GO) Nanosheets in Cultured HBE Cells

Overview

Journal Front Chem

Specialty Chemistry

Date 2022 May 26

PMID 35615314

Authors

Yule Zhang

Yatian Zhang

Zhijin Yang

Yan Fan

Mengya Chen

Mantong Zhao

Bo Dai

Lulu Zheng

Dawei Zhang

Affiliations

Soon will be listed here.

Abstract

Iron oxide (FeO), a classical magnetic material, has been widely utilized in the field of biological magnetic resonance imaging Graphene oxide (GO) has also been extensively applied as a drug carrier due to its high specific surface area and other properties. Recently, numerous studies have synthesized FeO/GO nanomaterials for biological diagnosis and treatments, including photothermal therapy and magnetic thermal therapy. However, the biosafety of the synthesized FeO/GO nanomaterials still needs to be further identified. Therefore, this research intended to ascertain the cytotoxicity of FeO/GO after treatment with different conditions in HBE cells. The results indicated the time-dependent and concentration-dependent cytotoxicity of FeO/GO. Meanwhile, exposure to FeO/GO nanomaterials increased reactive oxygen species (ROS) levels, calcium ions levels, and oxidative stress in mitochondria produced by these nanomaterials activated Caspase-9 and Caspase-3, ultimately leading to cell apoptosis.

Citing Articles

Nanocomposites Based on Iron Oxide and Carbonaceous Nanoparticles: From Synthesis to Their Biomedical Applications.

Vaduva M, Nila A, Udrescu A, Cramariuc O, Baibarac M Materials (Basel). 2025; 17(24.

PMID: 39769728 PMC: 11676432. DOI: 10.3390/ma17246127.

FeO Nanoparticles in Combination with 5-FU Exert Antitumor Effects Superior to Those of the Active Drug in a Colon Cancer Cell Model.

Genc S, Taghizadehghalehjoughi A, Yeni Y, Jafarizad A, Hacimuftuoglu A, Nikitovic D Pharmaceutics. 2023; 15(1).

PMID: 36678874 PMC: 9865889. DOI: 10.3390/pharmaceutics15010245.

References

Garcia-Salvador A, Katsumiti A, Rojas E, Aristimuno C, Betanzos M, Martinez-Moro M . A Complete In Vitro Toxicological Assessment of the Biological Effects of Cerium Oxide Nanoparticles: From Acute Toxicity to Multi-Dose Subchronic Cytotoxicity Study. Nanomaterials (Basel). 2021; 11(6). PMC: 8234921. DOI: 10.3390/nano11061577. View

Valdiglesias V . Cytotoxicity and Genotoxicity of Nanomaterials. Nanomaterials (Basel). 2022; 12(4). PMC: 8875473. DOI: 10.3390/nano12040634. View

Vannozzi L, Catalano E, Telkhozhayeva M, Teblum E, Yarmolenko A, Avraham E . Graphene Oxide and Reduced Graphene Oxide Nanoflakes Coated with Glycol Chitosan, Propylene Glycol Alginate, and Polydopamine: Characterization and Cytotoxicity in Human Chondrocytes. Nanomaterials (Basel). 2021; 11(8). PMC: 8399274. DOI: 10.3390/nano11082105. View

Zhang S, Wu S, Shen Y, Xiao Y, Gao L, Shi S . Cytotoxicity studies of FeO nanoparticles in chicken macrophage cells. R Soc Open Sci. 2020; 7(4):191561. PMC: 7211854. DOI: 10.1098/rsos.191561. View

Kaplan A, Kutlu H, Ciftci G . FeO Nanopowders: Genomic and Apoptotic Evaluations on A549 Lung Adenocarcinoma Cell Line. Nutr Cancer. 2019; 72(4):708-721. DOI: 10.1080/01635581.2019.1643031. View

Tang Z, Zhao L, Yang Z, Liu Z, Gu J, Bai B . Mechanisms of oxidative stress, apoptosis, and autophagy involved in graphene oxide nanomaterial anti-osteosarcoma effect. Int J Nanomedicine. 2018; 13:2907-2919. PMC: 5961647. DOI: 10.2147/IJN.S159388. View

Ahamed M, Akhtar M, Khan M . Investigation of Cytotoxicity, Apoptosis, and Oxidative Stress Response of FeO-RGO Nanocomposites in Human Liver HepG2 cells. Materials (Basel). 2020; 13(3). PMC: 7040707. DOI: 10.3390/ma13030660. View

Zhang H, Li S, Liu Y, Yu Y, Lin S, Wang Q . FeO@GO magnetic nanocomposites protect mesenchymal stem cells and promote osteogenic differentiation of rat bone marrow mesenchymal stem cells. Biomater Sci. 2020; 8(21):5984-5993. DOI: 10.1039/d0bm00906g. View

Chen X, Zhang X, Kubo H, Harris D, Mills G, Moyer J . Ca2+ influx-induced sarcoplasmic reticulum Ca2+ overload causes mitochondrial-dependent apoptosis in ventricular myocytes. Circ Res. 2005; 97(10):1009-17. DOI: 10.1161/01.RES.0000189270.72915.D1. View

10.

Wu Q, Yu R, Zhou Z, Liu H, Jiang R . Encapsulation of a Core-Shell Porous FeO@Carbon Material with Reduced Graphene Oxide for Li Battery Anodes with Long Cyclability. Langmuir. 2021; 37(2):785-792. DOI: 10.1021/acs.langmuir.0c03126. View

11.

Zhang Y, Han L, Qi W, Cheng D, Ma X, Hou L . Eicosapentaenoic acid (EPA) induced apoptosis in HepG2 cells through ROS-Ca(2+)-JNK mitochondrial pathways. Biochem Biophys Res Commun. 2014; 456(4):926-32. DOI: 10.1016/j.bbrc.2014.12.036. View

12.

Datta D, Khatri P, Singh A, Saha D, Verma G, Raman R . -induced ER-Mitochondrial calcium dynamics promotes calpain/caspase-12/caspase-9 mediated apoptosis in fish macrophages. Cell Death Discov. 2018; 4:30. PMC: 5841318. DOI: 10.1038/s41420-018-0034-9. View

13.

Wang X, Chang C, Jiang J, Liu X, Li J, Liu Q . Mechanistic Differences in Cell Death Responses to Metal-Based Engineered Nanomaterials in Kupffer Cells and Hepatocytes. Small. 2020; 16(21):e2000528. PMC: 7263057. DOI: 10.1002/smll.202000528. View

14.

Li D, Deng M, Yu Z, Liu W, Zhou G, Li W . Biocompatible and Stable GO-Coated FeO Nanocomposite: A Robust Drug Delivery Carrier for Simultaneous Tumor MR Imaging and Targeted Therapy. ACS Biomater Sci Eng. 2021; 4(6):2143-2154. DOI: 10.1021/acsbiomaterials.8b00029. View

15.

Zheng L, Zhang Y, Lin H, Kang S, Li Y, Sun D . Ultrasound and Near-Infrared Light Dual-Triggered Upconversion Zeolite-Based Nanocomposite for Hyperthermia-Enhanced Multimodal Melanoma Therapy via a Precise Apoptotic Mechanism. ACS Appl Mater Interfaces. 2020; 12(29):32420-32431. DOI: 10.1021/acsami.0c07297. View

16.

Zakharova O, Mastalygina E, Golokhvast K, Gusev A . Graphene Nanoribbons: Prospects of Application in Biomedicine and Toxicity. Nanomaterials (Basel). 2021; 11(9). PMC: 8469389. DOI: 10.3390/nano11092425. View

17.

Chen W, Yi P, Zhang Y, Zhang L, Deng Z, Zhang Z . Composites of aminodextran-coated Fe3O4 nanoparticles and graphene oxide for cellular magnetic resonance imaging. ACS Appl Mater Interfaces. 2011; 3(10):4085-91. DOI: 10.1021/am2009647. View

18.

Chen H, Xing L, Guo H, Luo C, Zhang X . Dual-targeting SERS-encoded graphene oxide nanocarrier for intracellular co-delivery of doxorubicin and 9-aminoacridine with enhanced combination therapy. Analyst. 2021; 146(22):6893-6901. DOI: 10.1039/d1an01237a. View

19.

Qiang S, Li Z, Zhang L, Luo D, Geng R, Zeng X . Cytotoxic Effect of Graphene Oxide Nanoribbons on . Nanomaterials (Basel). 2021; 11(5). PMC: 8160729. DOI: 10.3390/nano11051339. View

20.

Yan F, Liu Z, Zhang T, Zhang Q, Chen Y, Xie Y . Biphasic Injectable Bone Cement with FeO/GO Nanocomposites for the Minimally Invasive Treatment of Tumor-Induced Bone Destruction. ACS Biomater Sci Eng. 2021; 5(11):5833-5843. DOI: 10.1021/acsbiomaterials.9b00472. View