Photoacoustic Imaging of 3D-printed Vascular Networks

Overview

Journal Biofabrication

Date 2022 Jan 10

PMID 35008080

Authors

Chenshuo Ma

Wanlu Li

Daiwei Li

Maomao Chen

Mian Wang

Laiming Jiang

Luis Santiago Mille

Carlos Ezio Garciamendez

Zhibo Zhao

Qifa Zhou

Yu Shrike Zhang

Junjie Yao

Affiliations

Soon will be listed here.

Abstract

Thrombosis in the circulation system can lead to major myocardial infarction and cardiovascular deaths. Understanding thrombosis formation is necessary for developing safe and effective treatments. In this work, using digital light processing (DLP)-based 3D printing, we fabricated sophisticatedmodels of blood vessels with internal microchannels that can be used for thrombosis studies. In this regard, photoacoustic microscopy (PAM) offers a unique advantage for label-free visualization of the 3D-printed vessel models, with large penetration depth and functional sensitivity. We compared the imaging performances of two PAM implementations: optical-resolution PAM and acoustic-resolution PAM, and investigated 3D-printed vessel structures with different patterns of microchannels. Our results show that PAM can provide clear microchannel structures at depths up to 3.6 mm. We further quantified the blood oxygenation in the 3D-printed vascular models, showing that thrombi had lower oxygenation than the normal blood. We expect that PAM can find broad applications in 3D printing and bioprinting forstudies of various vascular and other diseases.

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