C2cd6-encoded CatSperτ Targets Sperm Calcium Channel to Ca Signaling Domains in the Flagellar Membrane

Overview

Journal Cell Rep

Publisher Cell Press

Specialties Biology
Cell Biology
Molecular Biology

Date 2022 Jan 9

PMID 34998468

Citations 16

Authors

Jae Yeon Hwang

Huafeng Wang

Yonggang Lu

Masahito Ikawa

Jean-Ju Chung

Affiliations

Soon will be listed here.

Abstract

In mammalian sperm cells, regulation of spatiotemporal Ca signaling relies on the quadrilinear Ca signaling nanodomains in the flagellar membrane. The sperm-specific, multi-subunit CatSper Ca channel, which is crucial for sperm hyperactivated motility and male fertility, organizes the nanodomains. Here, we report CatSperτ, the C2cd6-encoded membrane-associating C2 domain protein, can independently migrate to the flagella and serve as a major targeting component of the CatSper channel complex. CatSperτ loss of function in mice demonstrates that it is essential for sperm hyperactivated motility and male fertility. CatSperτ targets the CatSper channel into the quadrilinear nanodomains in the flagella of developing spermatids, whereas it is dispensable for functional channel assembly. CatSperτ interacts with ciliary trafficking machinery in a C2-dependent manner. These findings provide insights into the CatSper channel trafficking to the Ca signaling nanodomains and the shared molecular mechanisms of ciliary and flagellar membrane targeting.

Citing Articles

CATSPERε extracellular domains are essential for sperm calcium channel assembly and activity modulation.

Hwang J, Wang H, Clouser G, Oh J, Finnegan S, Skakkebaek N bioRxiv. 2024; .

PMID: 39605618 PMC: 11601665. DOI: 10.1101/2024.11.18.624146.

Sperm hyperactivation and the CatSper channel: current understanding and future contribution of domestic animals.

Hwang J J Anim Sci Technol. 2024; 66(3):443-456.

PMID: 38975583 PMC: 11222122. DOI: 10.5187/jast.2023.e133.

Ca homeostasis and male fertility: a target for a new male contraceptive system.

Lee K, Hwang J Anim Cells Syst (Seoul). 2024; 28(1):171-183.

PMID: 38686363 PMC: 11057403. DOI: 10.1080/19768354.2024.2345647.

Analysis of Ca-mediated sperm motility to evaluate the functional normality of the sperm-specific Ca channel, CatSper.

Hwang J Front Cell Dev Biol. 2024; 12:1284988.

PMID: 38385023 PMC: 10879342. DOI: 10.3389/fcell.2024.1284988.

Human fertilization in vivo and in vitro requires the CatSper channel to initiate sperm hyperactivation.

Young S, Schiffer C, Wagner A, Patz J, Potapenko A, Herrmann L J Clin Invest. 2024; 134(1).

PMID: 38165034 PMC: 10760960. DOI: 10.1172/JCI173564.

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