Perfluorocarbon Nanoemulsions Enhance Therapeutic SiRNA Delivery in the Treatment of Pulmonary Fibrosis

Overview

Journal Adv Sci (Weinh)

Specialty Biomedical Engineering

Date 2022 Jan 7

PMID 34994102

Citations 9

Authors

Ling Ding

Siyuan Tang

Weimin Tang

Deanna D Mosley

Ao Yu

Diptesh Sil

Svetlana Romanova

Kristina L Bailey

Daren L Knoell

Todd A Wyatt

David Oupicky

Affiliations

Soon will be listed here.

Abstract

Local pulmonary administration of therapeutic siRNA represents a promising approach to the treatment of lung fibrosis, which is currently hampered by inefficient delivery. Development of perfluorooctylbromide (PFOB) nanoemulsions as a way of improving the efficiency of pulmonary polycation-based delivery of siRNA is reported. The results show that the polycation/siRNA/PFOB nanoemulsions are capable of efficiently silencing the expression of STAT3 and inhibiting chemokine receptor CXCR4-two validated targets in pulmonary fibrosis. Both in vitro and in vivo results demonstrate that the nanoemulsions improve mucus penetration and facilitate effective cellular delivery of siRNA. Pulmonary treatment of mice with bleomycin-induced pulmonary fibrosis shows strong inhibition of the progression of the disease and significant prolongation of animal survival. Overall, the study points to a promising local treatment strategy of pulmonary fibrosis.

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