The Prevalence of Shiga Toxin-Producing and Enteropathogenic Isolated from Raw Chicken Meat Samples

Overview

Journal Int J Microbiol

Publisher Wiley

Specialty Microbiology

Date 2022 Jan 6

PMID 34987586

Citations 6

Authors

Omid Zarei

Leili Shokoohizadeh

Hadi Hossainpour

Mohammad Yousef Alikhani

Affiliations

Soon will be listed here.

Abstract

Background: Shiga toxin-producing (STEC) is known as a crucial zoonotic food-borne pathogen. A total of 257 raw chicken meat samples were collected from different markets in Hamadan, west of Iran, from January 2016 to May 2017.

Materials And Methods: The samples were cultured in selective and differential culture media, and the virulence genes of isolates were analyzed by PCR assay. The antibiotic resistance patterns of isolates were determined by the disk diffusion method. The genetic relatedness of the O157 isolates was analyzed by ERIC-PCR.

Results: In total, 93 (36% ± 3.12) of the isolates were identified as in this study. Based on serological and microbiological tests, 36 (38.7% ± 9.9), 7 (7.5% ± 5.35), and 12 (12.9% ± 6.81) of the isolates were characterized as STEC, enteropathogenic (EPEC), and attaching and effacing (AEEC) strains, respectively. A high level of resistance to nalidixic acid (91.4% ± 5.7), tetracycline (89.2% ± 6.31), ampicillin (82.8% ± 7.67), and trimotoprime-sulfametoxazole (71% ± 9.22) was detected among the isolates. The analysis of the ERIC-PCR results showed five different ERIC types among the O157 isolates.

Conclusions: Based on our findings, control and check-up of poultry meats should be considered as a crucial issue for public health.

Citing Articles

Molecular Detection of Shiga Toxin-Producing O177 Isolates, Their Antibiotic Resistance, and Virulence Profiles From Broiler Chickens.

Ramatla T, Motlhaping T, Ndlovu N, Mileng K, Howard J, Khasapane G Int J Microbiol. 2024; 2024:9778058.

PMID: 39665069 PMC: 11634401. DOI: 10.1155/ijm/9778058.

Prevalence, Molecular Characterization, and Antimicrobial Resistance Profiles of Shiga Toxin-Producing Isolated from Raw Beef, Pork, and Chicken Meat in Vietnam.

Duc H, Ha C, Hoa T, Hung L, Thang N, Son H Foods. 2024; 13(13).

PMID: 38998565 PMC: 11241076. DOI: 10.3390/foods13132059.

Plasmid-mediated quinolone resistance in isolates from commercial broiler chickens in Semnan, Iran.

Javadi B, Kafshdouzan K, Emadi Chashmi S, Pazhand O Iran J Microbiol. 2024; 16(2):193-200.

PMID: 38854977 PMC: 11162176. DOI: 10.18502/ijm.v16i2.15352.

Factors associated with microbiological contamination of chicken meat marketed in El Salvador.

Lopez A, Burgos T, Vanegas M, Alvarez Z, Mendez Y, Quinteros E Rev Peru Med Exp Salud Publica. 2023; 40(1):25-33.

PMID: 37377232 PMC: 10953630. DOI: 10.17843/rpmesp.2023.401.12100.

Knowledge, Hygienic Practices, and Toxi-Infectious Risks Associated with Ready-to-Eat Gbeli: A Particular Chip Derived from Cassava ( Crantz) Tuber Vended in Streets of Abomey-Calavi Municipality, Benin.

Noumavo A, Ohin M, Fadipe I, Hadji B, Ahouangansi S, Akin Y Biomed Res Int. 2022; 2022:8399831.

PMID: 36440363 PMC: 9691301. DOI: 10.1155/2022/8399831.

References

Dutta T, Roychoudhury P, Bandyopadhyay S, Wani S, Hussain I . Detection & characterization of Shiga toxin producing Escherichia coli (STEC) & enteropathogenic Escherichia coli (EPEC) in poultry birds with diarrhoea. Indian J Med Res. 2011; 133:541-5. PMC: 3121287. View

Momtaz H, Jamshidi A . Shiga toxin-producing Escherichia coli isolated from chicken meat in Iran: serogroups, virulence factors, and antimicrobial resistance properties. Poult Sci. 2013; 92(5):1305-13. DOI: 10.3382/ps.2012-02542. View

Pasquali F, Palma F, Trevisani M, Parisi A, Lucchi A, De Cesare A . Whole genome sequencing based typing and characterisation of Shiga-toxin producing strains belonging to O157 and O26 serotypes and isolated in dairy farms. Ital J Food Saf. 2019; 7(4):7673. PMC: 6379698. DOI: 10.4081/ijfs.2018.7673. View

Zarei O, Shokoohizadeh L, Hossainpour H, Alikhani M . The Prevalence of Shiga Toxin-Producing and Enteropathogenic Isolated from Raw Chicken Meat Samples. Int J Microbiol. 2022; 2021:3333240. PMC: 8723884. DOI: 10.1155/2021/3333240. View

Sekhar M, Sharif N, Rao T, Metta M . Genotyping of virulent obtained from poultry and poultry farm workers using enterobacterial repetitive intergenic consensus-polymerase chain reaction. Vet World. 2017; 10(11):1292-1296. PMC: 5732331. DOI: 10.14202/vetworld.2017.1292-1296. View

Lee G, Jang H, Hwang I, Rhee M . Prevalence and classification of pathogenic Escherichia coli isolated from fresh beef, poultry, and pork in Korea. Int J Food Microbiol. 2009; 134(3):196-200. DOI: 10.1016/j.ijfoodmicro.2009.06.013. View

Roth N, Kasbohrer A, Mayrhofer S, Zitz U, Hofacre C, Domig K . The application of antibiotics in broiler production and the resulting antibiotic resistance in Escherichia coli: A global overview. Poult Sci. 2018; 98(4):1791-1804. PMC: 6414035. DOI: 10.3382/ps/pey539. View

Frohlicher E, Krause G, Zweifel C, Beutin L, Stephan R . Characterization of attaching and effacing Escherichia coli (AEEC) isolated from pigs and sheep. BMC Microbiol. 2008; 8:144. PMC: 2571105. DOI: 10.1186/1471-2180-8-144. View

Bouzari S, Farhang E, Hosseini S, Alikhani M . Prevalence and antimicrobial resistance of shiga toxin-producing and enteropathogenic isolated from patients with acute diarrhea. Iran J Microbiol. 2018; 10(3):151-157. PMC: 6087697. View

10.

Trabulsi L, Keller R, Tardelli Gomes T . Typical and atypical enteropathogenic Escherichia coli. Emerg Infect Dis. 2002; 8(5):508-13. PMC: 2732489. DOI: 10.3201/eid0805.010385. View

11.

Zarei O, Shokoohizadeh L, Hossainpour H, Alikhani M . Molecular analysis of Pseudomonas aeruginosa isolated from clinical, environmental and cockroach sources by ERIC-PCR. BMC Res Notes. 2018; 11(1):668. PMC: 6139122. DOI: 10.1186/s13104-018-3765-z. View

12.

Zamani A, Yousefi Mashouf R, Ebrahimzadeh Namvar A, Alikhani M . Detection of magA Gene in Klebsiella spp. Isolated from Clinical SamplesDetection of magA. Iran J Basic Med Sci. 2013; 16(2):173-6. PMC: 3843861. View

13.

Exeni R, Fernandez-Brando R, Santiago A, Fiorentino G, Exeni A, Ramos M . Pathogenic role of inflammatory response during Shiga toxin-associated hemolytic uremic syndrome (HUS). Pediatr Nephrol. 2018; 33(11):2057-2071. DOI: 10.1007/s00467-017-3876-0. View

14.

Beutin L, Marches O, Bettelheim K, Gleier K, Zimmermann S, Schmidt H . HEp-2 cell adherence, actin aggregation, and intimin types of attaching and effacing Escherichia coli strains isolated from healthy infants in Germany and Australia. Infect Immun. 2003; 71(7):3995-4002. PMC: 162008. DOI: 10.1128/IAI.71.7.3995-4002.2003. View

15.

Fagan P, Hornitzky M, Bettelheim K, Djordjevic S . Detection of shiga-like toxin (stx1 and stx2), intimin (eaeA), and enterohemorrhagic Escherichia coli (EHEC) hemolysin (EHEC hlyA) genes in animal feces by multiplex PCR. Appl Environ Microbiol. 1999; 65(2):868-72. PMC: 91113. DOI: 10.1128/AEM.65.2.868-872.1999. View

16.

Ferens W, Hovde C . Escherichia coli O157:H7: animal reservoir and sources of human infection. Foodborne Pathog Dis. 2010; 8(4):465-87. PMC: 3123879. DOI: 10.1089/fpd.2010.0673. View

17.

Parreira V, Arns C, Yano T . Virulence factors of avian Escherichia coli associated with swollen head syndrome. Avian Pathol. 2008; 27(2):148-54. DOI: 10.1080/03079459808419316. View