Western Blot Profiles, Lymph Node Ultrastructure and Viral Expression in HIV-infected Patients: a Correlative Study

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1987 Jun 1

PMID 3498577

Citations 3

Authors

P U Cameron

R L Dawkins

J A Armstrong

E Bonifacio

Affiliations

Soon will be listed here.

Abstract

Sequential immunoblotting was performed on 64 patients infected with human immunodeficiency virus (HIV). Antibody profiles were related to immune function, T subsets and clinical features. In 20 patients, lymph node biopsy revealed a relationship between progressive follicular destruction, low antibody titres and ultrastructural evidence of viral replication and accumulation. Retroviral particles, including budding profiles, were confined to labyrinths formed from hypertrophied follicular dendritic (FD) cells; in some cases, including those with AIDS, the labyrinths showed degenerative changes. The demonstration of high antibody levels in asymptomatic patients with an intact FD cell network and low virion load suggests that antibody may have a protective role in vivo. Analysis of lymph node ultrastructure allows assessment of viral load and FD cell morphology. When combined with immunoblotting, it may be possible to improve prognostic stratification of patients with HIV infection.

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References

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

Mandel T, Phipps R, ABBOT A, Tew J . The follicular dendritic cell: long term antigen retention during immunity. Immunol Rev. 1980; 53:29-59. DOI: 10.1111/j.1600-065x.1980.tb01039.x. View

Fernandez R, Mouradian J, Metroka C, Davis J . The prognostic value of histopathology in persistent generalized lymphadenopathy in homosexual men. N Engl J Med. 1983; 309(3):185-6. DOI: 10.1056/NEJM198307213090314. View

Sarngadharan M, Popovic M, Bruch L, Schupbach J, Gallo R . Antibodies reactive with human T-lymphotropic retroviruses (HTLV-III) in the serum of patients with AIDS. Science. 1984; 224(4648):506-8. DOI: 10.1126/science.6324345. View

Safai B, Sarngadharan M, Groopman J, Arnett K, Popovic M, Sliski A . Seroepidemiological studies of human T-lymphotropic retrovirus type III in acquired immunodeficiency syndrome. Lancet. 1984; 1(8392):1438-40. DOI: 10.1016/s0140-6736(84)91933-0. View

Armstrong J, Horne R . Follicular dendritic cells and virus-like particles in AIDS-related lymphadenopathy. Lancet. 1984; 2(8399):370-2. DOI: 10.1016/s0140-6736(84)90540-3. View

Schupbach J, Haller O, Vogt M, Luthy R, Joller H, Oelz O . Antibodies to HTLV-III in Swiss patients with AIDS and pre-AIDS and in groups at risk for AIDS. N Engl J Med. 1985; 312(5):265-70. DOI: 10.1056/NEJM198501313120502. View

Janossy G, Pinching A, Bofill M, Weber J, McLaughlin J, Ornstein M . An immunohistological approach to persistent lymphadenopathy and its relevance to AIDS. Clin Exp Immunol. 1985; 59(2):257-66. PMC: 1577142. View

Wood G, Turner R, Shiurba R, Eng L, Warnke R . Human dendritic cells and macrophages. In situ immunophenotypic definition of subsets that exhibit specific morphologic and microenvironmental characteristics. Am J Pathol. 1985; 119(1):73-82. PMC: 1888071. View

10.

Weiss R, Clapham P, Dalgleish A, Carne C, Weller I, Tedder R . Neutralization of human T-lymphotropic virus type III by sera of AIDS and AIDS-risk patients. Nature. 1985; 316(6023):69-72. DOI: 10.1038/316069a0. View

11.

Robert-Guroff M, Brown M, Gallo R . HTLV-III-neutralizing antibodies in patients with AIDS and AIDS-related complex. Nature. 1985; 316(6023):72-4. DOI: 10.1038/316072a0. View

12.

Heinen E, Radoux D, Simar L . Follicular dendritic cells isolated from human tonsils. Adv Exp Med Biol. 1985; 186:985-91. DOI: 10.1007/978-1-4613-2463-8_119. View

13.

Biggar R, Melbye M, Ebbesen P, Alexander S, Nielsen J, Sarin P . Variation in human T lymphotropic virus III (HTLV-III) antibodies in homosexual men: decline before onset of illness related to acquired immune deficiency syndrome (AIDS). Br Med J (Clin Res Ed). 1985; 291(6501):997-8. PMC: 1416905. DOI: 10.1136/bmj.291.6501.997. View

14.

Pahwa S, Pahwa R, Saxinger C, Gallo R, GOOD R . Influence of the human T-lymphotropic virus/lymphadenopathy-associated virus on functions of human lymphocytes: evidence for immunosuppressive effects and polyclonal B-cell activation by banded viral preparations. Proc Natl Acad Sci U S A. 1985; 82(23):8198-202. PMC: 391470. DOI: 10.1073/pnas.82.23.8198. View

15.

McDougal J, Kennedy M, Sligh J, Cort S, Mawle A, Nicholson J . Binding of HTLV-III/LAV to T4+ T cells by a complex of the 110K viral protein and the T4 molecule. Science. 1986; 231(4736):382-5. DOI: 10.1126/science.3001934. View

16.

Tenner-Racz K, Racz P, Bofill M, Dietrich M, Kern P, Weber J . HTLV-III/LAV viral antigens in lymph nodes of homosexual men with persistent generalized lymphadenopathy and AIDS. Am J Pathol. 1986; 123(1):9-15. PMC: 1888153. View