The Diagnostic Role of PD-1 CXCR5 Follicular Helper CD8 T Cell in Renal Allograft Dysfunction

Overview

Journal J Clin Lab Anal

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
Pathology

Date 2021 Dec 27

PMID 34957609

Citations 2

Authors

Ji-Wen Fan

Lin Yan

Xue-Qiao Wang

Ya-Mei Li

Yang-Juan Bai

Xiao-Qi Ou

Zheng-Li Wan

Yi Li

Affiliations

Soon will be listed here.

Abstract

Background: The roles of PD-1 CXCR5 follicular helper CD8 T cell were reported in different disease conditions, but their roles in transplantation are unclear. In this study, the association between PD-1 CXCR5 follicular helper CD8 T cell and renal allograft dysfunction in kidney transplant recipients (KTRs) was investigated.

Methods: 82 KTRs were enrolled in this study. 45 KTRs were included in the chronic allograft dysfunction (CAD) group, and 37 KTRs were included in the stable recipients group. Among the CAD group, 12 cases of antibody-mediated rejection (ABMR) and 4 cases of T cell-mediated rejection (TCMR) were diagnosed by biopsy. The percentage of CXCR5 CD8 T cells and the co-expression of signal transducers and activators of transcription 4 (STAT4), STAT5, and PD-1 in peripheral blood were determined by flow cytometry.

Results: The expression of CXCR5 on CD3 CD8 T cells and the percentage of STAT5 CXCR5 cells in the CD3 CD8 T-cell population were significantly lower in the CAD group (p < 0.05), while the expression of PD-1 CXCR5 CD8 T cells was significantly higher (p < 0.05). Through logistic regression analysis, we concluded that the percentage of PD-1 CXCR5 CD8 T cells was an independent risk factor for renal dysfunction. Grouping by pathological type, PD-1 CXCR5 CD8 T cells showed relatively good diagnostic efficacy for ABMR by ROC analysis.

Conclusions: Our results suggested that PD-1 CXCR5 CD8 T cells were a promising biomarker for distinguishing renal allograft dysfunction and different allograft pathological types. Also, our findings may provide new ways of identifying and treating allograft rejection.

Citing Articles

Immune surveillance and humoral immune responses in kidney transplantation - A look back at T follicular helper cells.

Subburayalu J Front Immunol. 2023; 14:1114842.

PMID: 37503334 PMC: 10368994. DOI: 10.3389/fimmu.2023.1114842.

The diagnostic role of PD-1 CXCR5 follicular helper CD8 T cell in renal allograft dysfunction.

Fan J, Yan L, Wang X, Li Y, Bai Y, Ou X J Clin Lab Anal. 2021; 36(2):e24200.

PMID: 34957609 PMC: 8842189. DOI: 10.1002/jcla.24200.

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