Recombinant Murine Granulocyte Macrophage Colony-stimulating Factor Has Megakaryocyte Colony-stimulating Activity and Augments Megakaryocyte Colony Stimulation by Interleukin 3

Overview

Journal J Clin Invest

Specialty General Medicine

Date 1987 Jun 1

PMID 3495551

Citations 5

Authors

B E Robinson

H E McGrath

P J Quesenberry

Affiliations

Soon will be listed here.

Abstract

Recombinant murine granulocyte macrophage colony-stimulating factor (rGM-CSF) has been produced in Escherichia coli and purified to homogeneity. GM-CSF has an established role as an in vitro regulator of granulocyte and macrophage colony formation. We have determined that rGM-CSF also has intrinsic activity as a megakaryocyte colony-stimulating factor and that rGM-CSF augments the effect of interleukin 3 (IL-3) on megakaryocyte colony formation. The dose-response curve for megakaryocyte colony induction with rGM-CSF showed plateau megakaryocyte stimulation at 9 ng/ml. When IL-3 (at a plateau dose for megakaryocyte colony induction) was added to rGM-CSF over a 0-22-ng/ml dose range, the resultant megakaryocyte colony stimulation approximated the sum of the levels of stimulation produced by either factor alone. These results establish GM-CSF as a multilineage growth factor with definite megakaryocyte colony-stimulating activity and indicate that both GM-CSF and IL-3 are important in the regulation of megakaryocytopoiesis.

Citing Articles

Recombinant Granulocyte-Macrophage Colony-Stimulating Factor (rGM-CSF) : A Review of its Pharmacological Properties and Prospective Role in the Management of Myelosuppression.

Grant S, Heel R Drugs. 2017; 43(4):516-560.

PMID: 28421558 DOI: 10.2165/00003495-199243040-00008.

Human recombinant granulocyte-macrophage colony stimulating factor and interleukin 3 have overlapping but distinct hematopoietic activities.

Emerson S, Yang Y, Clark S, Long M J Clin Invest. 1988; 82(4):1282-7.

PMID: 3049674 PMC: 442680. DOI: 10.1172/JCI113727.

Human interleukin 6 is a direct promoter of maturation of megakaryocytes in vitro.

Ishibashi T, Kimura H, Uchida T, KARIYONE S, Friese P, BURSTEIN S Proc Natl Acad Sci U S A. 1989; 86(15):5953-7.

PMID: 2788282 PMC: 297749. DOI: 10.1073/pnas.86.15.5953.

The role of growth factors in haemopoietic development: clinical and biological implications.

Daniel C, Dexter T Cancer Metastasis Rev. 1989; 8(3):253-62.

PMID: 2697473 DOI: 10.1007/BF00047340.

Numerous growth factors can influence in vitro megakaryocytopoiesis.

Hoffman R, Briddell R, Bruno E Yale J Biol Med. 1990; 63(5):411-8.

PMID: 2293500 PMC: 2589366.

References

Jackson C . Cholinesterase as a possible marker for early cells of the megakaryocytic series. Blood. 1973; 42(3):413-21. View

McLeod D, Shreve M, AXELRAD A . Induction of megakaryocyte colonies with platelet formation in vitro. Nature. 1976; 261(5560):492-4. DOI: 10.1038/261492a0. View

Vainchenker W, Bouguet J, Guichard J, BRETON-GORIUS J . Megakaryocyte colony formation from human bone marrow precursors. Blood. 1979; 54(4):940-5. View

Williams N, Jackson H, Ralph P, Nakoinz I . Cell interactions influencing murine marrow megakaryocytes: nature of the potentiator cell in bone marrow. Blood. 1981; 57(1):157-63. View

Williams N, Eger R, JACKSON H, Nelson D . Two-factor requirement for murine megakaryocyte colony formation. J Cell Physiol. 1982; 110(1):101-4. DOI: 10.1002/jcp.1041100116. View

Levin J, LEVIN F, Hull 3rd D, Penington D . The effects of thrombopoietin on megakaryocyte-cfc, megakaryocytes, and thrombopoiesis: with studies of ploidy and platelet size. Blood. 1982; 60(4):989-98. View

Long M, Williams N, McDONALD T . Immature megakaryocytes in the mouse: in vitro relationship to megakaryocyte progenitor cells and mature megakaryocytes. J Cell Physiol. 1982; 112(3):339-44. DOI: 10.1002/jcp.1041120305. View

Ihle J, Keller J, Henderson L, Klein F, Palaszynski E . Procedures for the purification of interleukin 3 to homogeneity. J Immunol. 1982; 129(6):2431-6. View

Gualtieri R, Shadduck R, Baker D, Quesenberry P . Hematopoietic regulatory factors produced in long-term murine bone marrow cultures and the effect of in vitro irradiation. Blood. 1984; 64(2):516-25. View

10.

Williams N, Jackson H, Iscove N, DUKES P . The role of erythropoietin, thrombopoietic stimulating factor, and myeloid colony-stimulating factors on murine megakaryocyte colony formation. Exp Hematol. 1984; 12(9):734-40. View

11.

Quesenberry P, Ihle J, McGrath E . The effect of interleukin 3 and GM-CSA-2 on megakaryocyte and myeloid clonal colony formation. Blood. 1985; 65(1):214-7. View

12.

LUGARO G, Bernasconi G, CASELLATO M . Effect of a seminal inhibin-like factor on in vivo FSH and LH uptake by rat testis. Cell Biol Int Rep. 1984; 8(10):811. DOI: 10.1016/0309-1651(84)90063-8. View

13.

DeLamarter J, Mermod J, Liang C, Eliason J, Thatcher D . Recombinant murine GM-CSF from E. coli has biological activity and is neutralized by a specific antiserum. EMBO J. 1985; 4(10):2575-81. PMC: 554546. DOI: 10.1002/j.1460-2075.1985.tb03973.x. View

14.

Williams N, Sparrow R, Gill K, Yasmeen D, McNiece I . Murine megakaryocyte colony stimulating factor: its relationship to interleukin 3. Leuk Res. 1985; 9(12):1487-96. DOI: 10.1016/0145-2126(85)90041-4. View

15.

Oon S, Williams N . Biochemical characterization of an in-vitro murine megakaryocyte growth activity: megakaryocyte potentiator. Leuk Res. 1986; 10(4):403-11. DOI: 10.1016/0145-2126(86)90070-6. View

16.

Ishibashi T, BURSTEIN S . Interleukin 3 promotes the differentiation of isolated single megakaryocytes. Blood. 1986; 67(5):1512-4. View

17.

DUKES P, Egrie J, Strickland T, Browne J, Lin F . Megakaryocyte colony stimulating activity of recombinant human and monkey erythropoietin. Prog Clin Biol Res. 1986; 215:105-9. View

18.

Sparrow R, Williams N . Megakaryocyte colony stimulating factor: its identity to interleukin-3. Prog Clin Biol Res. 1986; 215:123-8. View

19.

Koike K, Shimizu T, Miyake T, Ihle J, Ogawa M . Hemopoietic colony formation by mouse spleen cells in serum-free culture supported by purified erythropoietin and/or interleukin-3. Prog Clin Biol Res. 1986; 215:33-49. View

20.

Williams N, Oon S, Jackson H, Lim R . Studies on megakaryocyte potentiator: its production and some biochemical characteristics. Prog Clin Biol Res. 1986; 215:91-103. View