A New ICE Subfamily Integrative and Conjugative Element Responsible for Horizontal Transfer of Biphenyl and Salicylic Acid Catabolic Pathway in the PCB-Degrading Strain KF716

Overview

Journal Microorganisms

Specialty Microbiology

Date 2021 Dec 24

PMID 34946064

Citations 6

Authors

Jun Hirose

Takahito Watanabe

Taiki Futagami

Hidehiko Fujihara

Nobutada Kimura

Hikaru Suenaga

Masatoshi Goto

Akiko Suyama

Kensuke Furukawa

Affiliations

Soon will be listed here.

Abstract

Integrative and conjugative elements (ICEs) are chromosomally integrated self-transmissible mobile genetic elements. Although some ICEs are known to carry genes for the degradation of aromatic compounds, information on their genetic features is limited. We identified a new member of the ICE family carrying biphenyl catabolic genes and salicylic acid catabolic genes from the PCB-degrading strain KF716. The 117-kb ICEKF716 contains common core regions exhibiting homology with those of degradative ICE from B13 and ICE from sp. CIB. A comparison of the gene loci collected from the public database revealed that several putative ICEs from B6-2 JAB1, AN10 and 2A20 had highly conserved core regions with those of ICEKF716, along with the variable region that encodes the catabolic genes for biphenyl, naphthalene, toluene, or phenol. These data indicate that this type of ICE subfamily is ubiquitously distributed within aromatic compound-degrading bacteria. ICEKF716 was transferred from KF716 to PAO1 via a circular extrachromosomal intermediate form. In this study, we describe the structure and genetic features of ICEKF716 compared to other catabolic ICEs.

Citing Articles

Genetic and Functional Characterization of a Salicylate 1-monooxygenase Located on an Integrative and Conjugative Element (ICE) in Pseudomonas stutzeri AJR13.

Ivanovski I, Zylstra G J Microbiol. 2023; 61(12):1025-1032.

PMID: 38100000 DOI: 10.1007/s12275-023-00093-x.

Evolution of genetic architecture and gene regulation in biphenyl/PCB-degrading bacteria.

Fujihara H, Hirose J, Suenaga H Front Microbiol. 2023; 14:1168246.

PMID: 37350784 PMC: 10282184. DOI: 10.3389/fmicb.2023.1168246.

Comparative genomics of (): diversity, habitats, and biodegradation of aromatic compounds.

Salva-Serra F, Perez-Pantoja D, Donoso R, Jaen-Luchoro D, Fernandez-Juarez V, Engstrom-Jakobsson H Front Microbiol. 2023; 14:1159176.

PMID: 37275147 PMC: 10234333. DOI: 10.3389/fmicb.2023.1159176.

Diversity and Evolution of Integrative and Conjugative Elements Involved in Bacterial Aromatic Compound Degradation and Their Utility in Environmental Remediation.

Hirose J Microorganisms. 2023; 11(2).

PMID: 36838403 PMC: 9960961. DOI: 10.3390/microorganisms11020438.

Breaking the ICE: an easy workflow for identifying and analyzing integrative and conjugative elements in bacterial genomes.

Goncalves O, de Assis J, Santana M Funct Integr Genomics. 2022; 22(6):1139-1145.

PMID: 36149586 DOI: 10.1007/s10142-022-00903-2.

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