Formation of a ZnO Nanorods-patterned Coating with Strong Bactericidal Capability and Quantitative Evaluation of the Contribution of Nanorods-derived Puncture and ROS-derived Killing

Overview

Journal Bioact Mater

Specialty Biomedical Engineering

Date 2021 Dec 23

PMID 34938922

Citations 8

Authors

Jing Ye

Bo Li

Mei Li

Yufeng Zheng

Shuilin Wu

Yong Han

Affiliations

Soon will be listed here.

Abstract

To endow Ti-based orthopedic implants with strong bactericidal activity, a ZnO nanorods-patterned coating (namely ZNR) was fabricated on Ti utilizing a catalyst- and template-free method of micro-arc oxidation (MAO) and hydrothermal treatment (HT). The coating comprises an outer layer of ZnO nanorods and a partially crystallized inner layer with nanocrystalline TiO and ZnTiO embedded amorphous matrix containing Ti, O and Zn. During HT, Zn ions contained in amorphous matrix of the as-MAOed layer migrate to surface and react with OH in hydrothermal solution to form ZnO nuclei growing in length at expense of the migrated Zn. ZNR exhibits intense bactericidal activity against the adhered and planktonic and . The crucial contributors to kill the adhered bacteria are ZnO nanorods derived mechano-penetration and released reactive oxygen species (ROS). Within 30 min of incubation, ROS is the predominant bactericidal contributor with quantitative contribution value of ∼20%, which transforms into mechano-penetration with prolonging time to reach quantitative contribution value of ∼96% at 24 h. In addition, the bactericidal contributor against the planktonic bacteria of ZNR is relied on the released Zn. This work discloses an in-depth bactericidal mechanism of ZnO nanorods.

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