Vitamin D Metabolic Pathway Genes Polymorphisms and Their Methylation Levels in Association With Rheumatoid Arthritis

Overview

Journal Front Immunol

Specialty Allergy & Immunology

Date 2021 Dec 20

PMID 34925313

Citations 7

Authors

Tian-Ping Zhang

Hong-Miao Li

Qian Huang

Li Wang

Xiao-Mei Li

Affiliations

Soon will be listed here.

Abstract

Abnormal vitamin D metabolism is involved in the pathogenesis of rheumatoid arthritis (RA). In this study, we evaluated the association of single nucleotide polymorphisms (SNPs) and methylation levels in vitamin D metabolic pathway genes with RA susceptibility. Ten SNPs in vitamin D metabolic pathway genes (, , , ) were genotyped in 477 RA patients and 496 controls by improved multiple ligase detection reaction (iMLDR). The methylation levels of the promoter regions of these genes were detected in 122 RA patients and 123 controls using Illumina Hiseq platform. We found that the rs1993116 GA genotype, rs4646536 GA genotype, rs4646536 A allele frequencies were significantly increased in RA patients when compared to controls. The decreased risk of rs1993116, rs4646536 was found under the dominant mode in RA patients. However, no significant association was found between rs7936142, rs12794714, rs2762934, rs6068816, rs2296239, rs2296241, rs11574129, rs3847987 polymorphism, and RA susceptibility. The , methylation levels in RA patients were significantly lower than those in controls, while , methylation levels were not associated with RA. There were no statistical associations between , , , methylation levels and their respective genotype in RA patients. In addition, plasma 25OHD level in RA patients was significantly lower than that in healthy controls. In summary, our results showed that , genetic variations were associated with the genetic background of RA, while altered , methylation levels were related to the risk of RA.

Citing Articles

Implications of vitamin D levels or status for mortality in rheumatoid arthritis: analysis of 2001-2018 data from the National Health and Nutrition Examination Survey.

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Methylation of the Vitamin D Receptor Gene in Human Disorders.

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Association of DNA methylation of vitamin D metabolic pathway related genes with colorectal cancer risk.

Wang Y, Li L, Deng X, Fang Y, Zhang C Clin Epigenetics. 2023; 15(1):140.

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Detecting potential mechanism of vitamin D in treating rheumatoid arthritis based on network pharmacology and molecular docking.

Xu X, Luo H, Chen Q, Wang Z, Chen X, Li X Front Pharmacol. 2022; 13:1047061.

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Vitamin D as a Nutri-Epigenetic Factor in Autoimmunity-A Review of Current Research and Reports on Vitamin D Deficiency in Autoimmune Diseases.

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