Characterization of Synovial T Lymphocytes in Rheumatoid Arthritis. I. Production of IL-2 Dependent T Cell Clones from Synovial Fluid and Peripheral Blood

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1986 Jun 1

PMID 3491696

Citations 6

Authors

W A Ofosu-Appiah

R M McKenna

R J Warrington

J A Wilkins

Affiliations

Soon will be listed here.

Abstract

Lymphocytes obtained from the peripheral blood (PBL) or synovial fluids (SFL) of patients with rheumatoid arthritis (RA) or other inflammatory joint diseases were compared with the PBL from normal individuals, by cloning under limiting dilution conditions in the presence of interleukin 2 (IL-2). The precursor frequency estimates of IL-2 responsive cells from these sources did not differ appreciably. However there were marked differences in the surface marker phenotypes of the clones derived from the PBL as compared to SFL. There was a predominance of OKT4-8+ cells in SFL from RA and non RA donors with inflammatory joint disease while PBL from all sources showed a marked prevalence of OKT4+8- cells. Comparison of precursor frequencies in the presence of PBL and SFL indicated that there were variations in the capacities of the SFL and PBL IL-2 dependent cells to grow on these fillers. SFL derived cells grew equally well on PBL or SFL filler, while PBL clones grew efficiently only on PBL fillers. Collectively these results indicate that there are marked differences in the surface phenotypes and growth requirements of IL-2 responsive SFL as compared to PBL.

Citing Articles

Increment of CD8S6F1 cells in synovial fluid from patients with rheumatoid arthritis.

Ueki Y, Eguchi K, Miyake S, Nagataki S, Tominaga Y Ann Rheum Dis. 1994; 53(12):816-22.

PMID: 7864690 PMC: 1005482. DOI: 10.1136/ard.53.12.816.

Characterization of IL-2 responsive synovial T lymphocytes in rheumatoid arthritis. II. Functional properties.

Ofosu-Appiah W, Warrington R, Wilkins J Rheumatol Int. 1987; 7(4):147-51.

PMID: 3499651 DOI: 10.1007/BF00270362.

Fibroblast-activating factor production by interleukin (IL)-2 dependent T-cell clones from rheumatoid arthritis patients and normal donors.

Ofosu-Appiah W, Warrington R, Wilkins J Rheumatol Int. 1988; 8(5):219-24.

PMID: 3266357 DOI: 10.1007/BF00269198.

Reversible interleukin-2 response defects in systemic lupus erythematosus.

Warrington R, SAUDER P, Homik J Clin Exp Immunol. 1989; 77(2):163-7.

PMID: 2789113 PMC: 1541987.

Immunoregulatory T cell subsets and T cell activation in rheumatoid arthritis. A need for analysis on the clonal and molecular level.

Quayle A, Kjeldsen-Kragh J, Forre O, Waalen K, Sioud M, Kalvenes C Springer Semin Immunopathol. 1989; 11(3):273-87.

PMID: 2575800 DOI: 10.1007/BF00197307.

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