The Wall-less Bacterium Builds a Polymeric Cytoskeleton Composed of Interacting MreB Isoforms

Overview

Journal iScience

Publisher Cell Press

Date 2021 Dec 10

PMID 34888500

Citations 10

Authors

Florent Masson

Xavier Pierrat

Bruno Lemaitre

Alexandre Persat

Affiliations

Soon will be listed here.

Abstract

A rigid cell wall defines the morphology of most bacteria. MreB, a bacterial homologue of actin, plays a major role in coordinating cell wall biogenesis and defining cell shape. are wall-less bacteria that robustly grow with a characteristic helical shape. Paradoxal to their lack of cell wall, the genome contains five homologs of MreB (SpMreBs). Here, we investigate the function of SpMreBs in forming a polymeric cytoskeleton. We found that, , maintain a high concentration of all MreB isoforms. By leveraging a heterologous expression system that bypasses the poor genetic tractability of , we found that SpMreBs produced polymeric filaments of various morphologies. We characterized an interaction network between isoforms that regulate filament formation and patterning. Therefore, our results support the hypothesis where combined SpMreB isoforms would form an inner polymeric cytoskeleton that shapes the cell in a wall-independent manner.

Citing Articles

Deciphering the impact of MreB on the morphology and pathogenicity of the aquatic pathogen Spiroplasma eriocheiris.

Li R, Cao X, Chen J, He T, Zhang Y, Wang W Biol Direct. 2024; 19(1):98.

PMID: 39444023 PMC: 11515736. DOI: 10.1186/s13062-024-00537-3.

Assembly properties of bacterial actin MreB involved in Spiroplasma swimming motility.

Takahashi D, Miyata M, Fujiwara I J Biol Chem. 2023; 299(6):104793.

PMID: 37150324 PMC: 10279915. DOI: 10.1016/j.jbc.2023.104793.

Sequence analyses of a lipoprotein conserved with bacterial actins responsible for swimming motility of wall-less helical .

Takahashi D, Miyata M MicroPubl Biol. 2023; 2023.

PMID: 37033705 PMC: 10074174. DOI: 10.17912/micropub.biology.000713.

Swimming Motility Assays of Spiroplasma.

Nakane D Methods Mol Biol. 2023; 2646:373-381.

PMID: 36842131 DOI: 10.1007/978-1-0716-3060-0_31.

Purification and ATPase Activity Measurement of Spiroplasma MreB.

Takahashi D, Fujiwara I, Miyata M Methods Mol Biol. 2023; 2646:359-371.

PMID: 36842130 DOI: 10.1007/978-1-0716-3060-0_30.

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