Coupled, Circumferential Motions of the Cell Wall Synthesis Machinery and MreB Filaments in B. Subtilis

Overview

Journal Science

Specialty Science

Date 2011 Jun 4

PMID 21636745

Citations 303

Authors

Ethan C Garner

Remi Bernard

Wenqin Wang

Xiaowei Zhuang

David Z Rudner

Tim Mitchison

Affiliations

Soon will be listed here.

Abstract

Rod-shaped bacteria elongate by the action of cell wall synthesis complexes linked to underlying dynamic MreB filaments. To understand how the movements of these filaments relate to cell wall synthesis, we characterized the dynamics of MreB and the cell wall elongation machinery using high-precision particle tracking in Bacillus subtilis. We found that MreB and the elongation machinery moved circumferentially around the cell, perpendicular to its length, with nearby synthesis complexes and MreB filaments moving independently in both directions. Inhibition of cell wall synthesis by various methods blocked the movement of MreB. Thus, bacteria elongate by the uncoordinated, circumferential movements of synthetic complexes that insert radial hoops of new peptidoglycan during their transit, possibly driving the motion of the underlying MreB filaments.

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