The Dynamics of Nucleoid Structure at the Exponential and Stationary Growth Phases

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2021 Dec 6

PMID 34867875

Citations 3

Authors

Gleb Y Fisunov

Alexander I Zubov

Olga V Pobeguts

Anna M Varizhuk

Mariya A Galyamina

Daria V Evsyutina

Tatiana A Semashko

Valentin A Manuvera

Sergey I Kovalchuk

Rustam K Ziganshin

Nicolay A Barinov

Dmitry V Klinov

Vadim M Govorun

Affiliations

Soon will be listed here.

Abstract

The structure and dynamics of bacterial nucleoids play important roles in regulating gene expression. Bacteria of class Mollicutes and, in particular, mycoplasmas feature extremely reduced genomes. They lack multiple structural proteins of the nucleoid, as well as regulators of gene expression. We studied the organization of nucleoids in the stationary and exponential growth phases at the structural and protein levels. The growth phase transition results in the structural reorganization of nucleoid. In particular, it undergoes condensation and changes in the protein content. The observed changes corroborate with the previously identified global rearrangement of the transcriptional landscape in this bacterium during the growth phase transition. In addition, we identified that the glycolytic enzyme enolase functions as a nucleoid structural protein in this bacterium. It is capable of non-specific DNA binding and can form fibril-like complexes with DNA.

Citing Articles

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