Detection of T Cell Receptor Alpha Chain Locus Rearrangements in Human Antigen-specific T Lymphocyte Clones Using a DNA Probe Specific for Chromosome Region 14q11

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1986 May 1

PMID 3486246

Authors

L K Chen

C J Larsen

A Bensussan

Affiliations

Soon will be listed here.

Abstract

Human cloned antigen-specific T lymphocytes were used to characterize DNA rearrangements using a cDNA probe from the T cell receptor (TCR)-alpha chain gene. Rearranged patterns were detected in some T cell clones, confirming that normal mature T cells are rearranged in TCR-alpha locus. Similarly, rearranged DNA patterns were found in T cell clones from the same panel, using a DNA probe (clone K-40, [1]) isolated from chromosome 14 (14q11), where the TCR-alpha locus has been mapped. These results suggest that this genomic DNA clone is located within the TCR-alpha chain locus.

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