Procainamide Elicits a Selective Autoantibody Immune Response

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1986 Jan 1

PMID 3485487

Citations 9

Authors

R L Rubin

G Reimer

E M MCNALLY

S R Nusinow

R P Searles

E M Tan

Affiliations

Soon will be listed here.

Abstract

The specificity of the in vivo humoral immune response elicited by procainamide was examined by solid-phase assays, immunofluorescence, immunoprecipitation and a cytotoxicity assay. Serial samples obtained from patients during their procainamide therapy showed a progressive increase in antibodies to histones and denatured DNA, and both activities decreased after discontinuation of therapy. In contrast antibodies to tetanus, human IgG (rheumatoid factor) and heterologous lymphocytes were unaffected by procainamide treatment, indicating that they were not drug-induced. Of 29 sera examined by protein-A-facilitated immunoprecipitation, four sera had antibody to ribosomal RNA and three sera immunoprecipitated a 40kD protein. Antinuclear antibodies were invariably present but absorption studies showed that these activities were due to anti-histone antibodies. These results indicate that procainamide-induced autoimmunity is characterized predominantly by an anti-histone and anti-denatured DNA immune response.

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References

Woosley R, Drayer D, Reidenberg M, Nies A, Carr K, OATES J . Effect of acetylator phenotype on the rate at which procainamide induces antinuclear antibodies and the lupus syndrome. N Engl J Med. 1978; 298(21):1157-9. DOI: 10.1056/NEJM197805252982101. View

Winfield J, Koffler D, KUNKEL H . Development of antibodies to ribonucleoprotein following short-term therapy with procainamide. Arthritis Rheum. 1975; 18(6):531-4. DOI: 10.1002/art.1780180601. View

Bluestein H, Zvaifler N, Weisman M, Shapiro R . Lymphocyte alteration by procainamide: relation to drug-induced lupus erythematosus syndrome. Lancet. 1979; 2(8147):816-9. DOI: 10.1016/s0140-6736(79)92174-3. View

Lerner M, Boyle J, Hardin J, Steitz J . Two novel classes of small ribonucleoproteins detected by antibodies associated with lupus erythematosus. Science. 1981; 211(4480):400-2. DOI: 10.1126/science.6164096. View

Grossman L, Barland P . Histone reactivity of drug-induced antinuclear antibodies. A comparison of symptomatic and asymptomatic patients. Arthritis Rheum. 1981; 24(7):927-31. DOI: 10.1002/art.1780240710. View

Goodwin J, Searles R, Tung K . Immunological responses of healthy elderly population. Clin Exp Immunol. 1982; 48(2):403-10. PMC: 1536458. View

Miller K, Salem D . Immune regulatory abnormalities produced by procainamide. Am J Med. 1982; 73(4):487-92. DOI: 10.1016/0002-9343(82)90326-6. View

Matter L, Schopfer K, Wilhelm J, Nyffenegger T, Parisot R, De Robertis E . Molecular characterization of ribonucleoprotein antigens bound by antinuclear antibodies. A diagnostic evaluation. Arthritis Rheum. 1982; 25(11):1278-83. DOI: 10.1002/art.1780251102. View

Ochi T, Goldings E, Lipsky P, ZIFF M . Immunomodulatory effect of procainamide in man. Inhibition of human suppressor T-cell activity in vitro. J Clin Invest. 1983; 71(1):36-45. PMC: 436835. DOI: 10.1172/jci110749. View

10.

Francoeur A, Mathews M . Interaction between VA RNA and the lupus antigen La: formation of a ribonucleoprotein particle in vitro. Proc Natl Acad Sci U S A. 1982; 79(22):6772-6. PMC: 347215. DOI: 10.1073/pnas.79.22.6772. View

11.

Portanova J, Rubin R, Joslin F, Agnello V, Tan E . Reactivity of anti-histone antibodies induced by procainamide and hydralazine. Clin Immunol Immunopathol. 1982; 25(1):67-79. DOI: 10.1016/0090-1229(82)90166-0. View

12.

Searles R, Okudaira K, Savage S, Goodwin J . Ia specific antilymphocyte antibodies in rheumatoid arthritis. Arthritis Rheum. 1983; 26(4):486-93. DOI: 10.1002/art.1780260406. View

13.

Klajman A, Kafri B, Shohat T, Drucker I, Moalem T, Jaretzky A . The prevalence of antibodies to histones induced by procainamide in old people, in cancer patients, and in rheumatoid-like disease. Clin Immunol Immunopathol. 1983; 27(1):1-8. DOI: 10.1016/0090-1229(83)90050-8. View

14.

Rubin R, Joslin F, Tan E . An improved ELISA for anti-native DNA by elimination of interference by anti-histone antibodies. J Immunol Methods. 1983; 63(3):359-66. DOI: 10.1016/s0022-1759(83)80009-x. View

15.

McNeilage L, Whittingham S, Mackay I . Autoantibodies reactive with small ribonucleoprotein antigens: a convergence of molecular biology and clinical immunology. J Clin Lab Immunol. 1984; 15(1):1-17. View

16.

Rubin R, MCNALLY E, Nusinow S, Robinson C, Tan E . IgG antibodies to the histone complex H2A-H2B characterize procainamide-induced lupus. Clin Immunol Immunopathol. 1985; 36(1):49-59. DOI: 10.1016/0090-1229(85)90038-8. View

17.

Koffler D, Carr R, Agnello V, Fiezi T, KUNKEL H . Antibodies to polynucleotides: distribution in human serums. Science. 1969; 166(3913):1648-9. DOI: 10.1126/science.166.3913.1648. View

18.

Molina J, DUBOIS E, Bilitch M, Bland S, Friou G . Procainamide-induced serologic changes in asymptomatic patients. Arthritis Rheum. 1969; 12(6):608-14. DOI: 10.1002/art.1780120608. View

19.

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

20.

Terasaki P, Mottironi V, Barnett E . Cytotoxins in disease. Autocytotoxins in lupus. N Engl J Med. 1970; 283(14):724-8. DOI: 10.1056/NEJM197010012831403. View