3D Bioprinting for Orthopaedic Applications: Current Advances, Challenges and Regulatory Considerations

Overview

Journal Bioprinting

Date 2021 Dec 2

PMID 34853818

Citations 12

Authors

D Stanco

P Urban

S Tirendi

G Ciardelli

J Barrero

Affiliations

Soon will be listed here.

Abstract

In the era of personalised medicine, novel therapeutic approaches raise increasing hopes to address currently unmet medical needs by developing patient-customised treatments. Three-dimensional (3D) bioprinting is rapidly evolving and has the potential to obtain personalised tissue constructs and overcome some limitations of standard tissue engineering approaches. Bioprinting could support a wide range of biomedical applications, such as drug testing, tissue repair or organ transplantation. There is a growing interest for 3D bioprinting in the orthopaedic field, with remarkable scientific and technical advances. However, the full exploitation of 3D bioprinting in medical applications still requires efforts to anticipate the upcoming challenges in translating bioprinted products from bench to bedside. In this review we summarised current trends, advances and challenges in the application of 3D bioprinting for bone and cartilage tissue engineering. Moreover, we provided a detailed analysis of the applicable regulations through the 3D bioprinting process and an overview of available standards covering bioprinting and additive manufacturing.

Citing Articles

Three-Dimensional Printing/Bioprinting and Cellular Therapies for Regenerative Medicine: Current Advances.

Sousa A, Alvites R, Lopes B, Sousa P, Moreira A, Coelho A J Funct Biomater. 2025; 16(1).

PMID: 39852584 PMC: 11765675. DOI: 10.3390/jfb16010028.

Bioprinting and Intellectual Property: Challenges, Opportunities, and the Road Ahead.

Kantaros A, Ganetsos T, Petrescu F, Alysandratou E Bioengineering (Basel). 2025; 12(1).

PMID: 39851350 PMC: 11761581. DOI: 10.3390/bioengineering12010076.

Current advances in the development of microRNA-integrated tissue engineering strategies: a cornerstone of regenerative medicine.

Castanon-Cortes L, Bravo-Vazquez L, Santoyo-Valencia G, Medina-Feria S, Sahare P, Duttaroy A Front Bioeng Biotechnol. 2024; 12:1484151.

PMID: 39479296 PMC: 11521876. DOI: 10.3389/fbioe.2024.1484151.

Three-Dimensional Bioprinting: A Comprehensive Review for Applications in Tissue Engineering and Regenerative Medicine.

Mirsky N, Ehlen Q, Greenfield J, Antonietti M, Slavin B, Nayak V Bioengineering (Basel). 2024; 11(8).

PMID: 39199735 PMC: 11351251. DOI: 10.3390/bioengineering11080777.

Strategies in product engineering of mesenchymal stem cell-derived exosomes: unveiling the mechanisms underpinning the promotive effects of mesenchymal stem cell-derived exosomes.

Jiang Y, Lv H, Shen F, Fan L, Zhang H, Huang Y Front Bioeng Biotechnol. 2024; 12:1363780.

PMID: 38756412 PMC: 11096451. DOI: 10.3389/fbioe.2024.1363780.

References

Fedorovich N, Wijnberg H, Dhert W, Alblas J . Distinct tissue formation by heterogeneous printing of osteo- and endothelial progenitor cells. Tissue Eng Part A. 2011; 17(15-16):2113-21. DOI: 10.1089/ten.TEA.2011.0019. View

Kang H, Lee S, Ko I, Kengla C, Yoo J, Atala A . A 3D bioprinting system to produce human-scale tissue constructs with structural integrity. Nat Biotechnol. 2016; 34(3):312-9. DOI: 10.1038/nbt.3413. View

Keriquel V, Oliveira H, Remy M, Ziane S, Delmond S, Rousseau B . In situ printing of mesenchymal stromal cells, by laser-assisted bioprinting, for in vivo bone regeneration applications. Sci Rep. 2017; 7(1):1778. PMC: 5431768. DOI: 10.1038/s41598-017-01914-x. View

Aljohani W, Ullah M, Zhang X, Yang G . Bioprinting and its applications in tissue engineering and regenerative medicine. Int J Biol Macromol. 2017; 107(Pt A):261-275. DOI: 10.1016/j.ijbiomac.2017.08.171. View

Daly A, Kelly D . Biofabrication of spatially organised tissues by directing the growth of cellular spheroids within 3D printed polymeric microchambers. Biomaterials. 2019; 197:194-206. DOI: 10.1016/j.biomaterials.2018.12.028. View

Cohen D, Malone E, Lipson H, Bonassar L . Direct freeform fabrication of seeded hydrogels in arbitrary geometries. Tissue Eng. 2006; 12(5):1325-35. DOI: 10.1089/ten.2006.12.1325. View

Demirtas T, Irmak G, Gumusderelioglu M . A bioprintable form of chitosan hydrogel for bone tissue engineering. Biofabrication. 2017; 9(3):035003. DOI: 10.1088/1758-5090/aa7b1d. View

Kundu J, Shim J, Jang J, Kim S, Cho D . An additive manufacturing-based PCL-alginate-chondrocyte bioprinted scaffold for cartilage tissue engineering. J Tissue Eng Regen Med. 2013; 9(11):1286-97. DOI: 10.1002/term.1682. View

Brittberg M, Lindahl A, Nilsson A, Ohlsson C, Isaksson O, Peterson L . Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. N Engl J Med. 1994; 331(14):889-95. DOI: 10.1056/NEJM199410063311401. View

10.

Fedorovich N, Schuurman W, Wijnberg H, Prins H, van Weeren P, Malda J . Biofabrication of osteochondral tissue equivalents by printing topologically defined, cell-laden hydrogel scaffolds. Tissue Eng Part C Methods. 2011; 18(1):33-44. PMC: 3245674. DOI: 10.1089/ten.TEC.2011.0060. View

11.

Kerouredan O, Hakobyan D, Remy M, Ziane S, Dusserre N, Fricain J . In situ prevascularization designed by laser-assisted bioprinting: effect on bone regeneration. Biofabrication. 2019; 11(4):045002. DOI: 10.1088/1758-5090/ab2620. View

12.

Peak C, Stein J, Gold K, Gaharwar A . Nanoengineered Colloidal Inks for 3D Bioprinting. Langmuir. 2017; 34(3):917-925. DOI: 10.1021/acs.langmuir.7b02540. View

13.

Wang X, Tolba E, Schroder H, Neufurth M, Feng Q, Diehl-Seifert B . Effect of bioglass on growth and biomineralization of SaOS-2 cells in hydrogel after 3D cell bioprinting. PLoS One. 2014; 9(11):e112497. PMC: 4226565. DOI: 10.1371/journal.pone.0112497. View

14.

Birru B, Mekala N, Parcha S . Mechanistic role of perfusion culture on bone regeneration. J Biosci. 2019; 44(1). View

15.

Ayyildiz-Tamis D, Avci K, Deliloglu-Gurhan S . Comparative investigation of the use of various commercial microcarriers as a substrate for culturing mammalian cells. In Vitro Cell Dev Biol Anim. 2013; 50(3):221-31. DOI: 10.1007/s11626-013-9717-y. View

16.

Tatara A, Mikos A . Tissue Engineering in Orthopaedics. J Bone Joint Surg Am. 2016; 98(13):1132-9. PMC: 4928040. DOI: 10.2106/JBJS.16.00299. View

17.

Barron M, Goldman J, Tsai C, Donahue S . Perfusion flow enhances osteogenic gene expression and the infiltration of osteoblasts and endothelial cells into three-dimensional calcium phosphate scaffolds. Int J Biomater. 2012; 2012:915620. PMC: 3440867. DOI: 10.1155/2012/915620. View

18.

Yang X, Lu Z, Wu H, Li W, Zheng L, Zhao J . Collagen-alginate as bioink for three-dimensional (3D) cell printing based cartilage tissue engineering. Mater Sci Eng C Mater Biol Appl. 2017; 83:195-201. DOI: 10.1016/j.msec.2017.09.002. View

19.

Cidonio G, Glinka M, Kim Y, Kanczler J, Lanham S, Ahlfeld T . Nanoclay-based 3D printed scaffolds promote vascular ingrowth ex vivo and generate bone mineral tissue in vitro and in vivo. Biofabrication. 2020; 12(3):035010. DOI: 10.1088/1758-5090/ab8753. View

20.

Gomes M, Rodrigues M, Domingues R, Reis R . Tissue Engineering and Regenerative Medicine: New Trends and Directions-A Year in Review. Tissue Eng Part B Rev. 2017; 23(3):211-224. DOI: 10.1089/ten.TEB.2017.0081. View