The Potential of Sperm Bovine Protamine As a Protein Marker of Semen Production and Quality at the National Artificial Insemination Center of Indonesia

Overview

Journal Vet World

Specialty Veterinary Medicine

Date 2021 Nov 29

PMID 34840468

Citations 7

Authors

Berlin Pandapotan Pardede

Tulus Maulana

Ekayanti Mulyawati Kaiin

Muhammad Agil

Ni Wayan Kurniani Karja

Cece Sumantri

Iman Supriatna

Affiliations

Soon will be listed here.

Abstract

Background And Aim: Protamine (PRM) is the major protein in the sperm nucleus and plays an essential role in its normal function. Moreover, PRM has great potential as a protein marker of semen production and quality. This study aimed to assess the potential of sperm bovine PRM as a protein marker of semen production and quality in bulls at the National Artificial Insemination (AI) Center of Indonesia.

Materials And Methods: The semen production capacity of each bull was collected from frozen semen production data at the Singosari AI Center for 6 months, and was then divided into two groups (high and low). A total of 440 frozen semen straws from six Limousin (LIM), six Friesian Holstein (FH), six Peranakan Ongole (PO), and four Aceh bulls aged 4-5 years were used in the study. The frozen semen was used to measure the concentration of PRM1, PRM2, and PRM3 using the enzyme immunoassay method. The frozen semen was also used to assess the quality of the semen, including progressive motility (PM) through computer-assisted semen analysis, sperm viability through eosin-nigrosin analysis, and the DNA fragmentation index through Acridine Orange staining.

Results: PRM1 was significantly higher in all bull breeds included in the study (p<0.00), followed by PRM2 (p<0.00) and PRM3 (p<0.00). PRM1 significantly affected semen production in LIM, FH, PO, and Aceh bulls (p<0.05). Moreover, PRM2 significantly affected semen production only in FH and Aceh bulls (p<0.05), whereas PRM3 affected this parameter in PO and Aceh bulls exclusively (p<0.05). Consistently and significantly, PRM1 was positively correlated with the PM and viability of sperm and negatively associated with its DNA fragmentation in LIM, FH, PO, and Aceh bulls (p<0.05; p<0.01). The correlation analysis between PRM2 and PRM3 and semen quality parameters varied across all bull breeds; some were positively and negatively correlated (p<0.05; p<0.01), and some were not correlated at all.

Conclusion: PRM1 has excellent potential as a protein marker of semen production and quality in bulls at the National AI Center of Indonesia.

Citing Articles

A-Kinase Anchor Protein 4 (proAKAP4): Protein Molecule-Based Fertility Marker of Indonesian Dairy Bull and Its Correlation With Frozen-Thawed Sperm Quality.

Pardede B, Setyawan E, Said S, Kusumawati A, Purwantara B, Pangestu M Vet Med Int. 2025; 2025:8367714.

PMID: 40041135 PMC: 11876519. DOI: 10.1155/vmi/8367714.

The frozen-thawed sperm protein of Indonesian Toraya buffaloes is significantly associated with sperm kinematics, acrosome integrity, and mitochondrial membrane potential.

Maulana T, Said S, Arifiantini R, Jakaria J, Gunawan A J Adv Vet Anim Res. 2025; 11(4):869-879.

PMID: 40013284 PMC: 11855436. DOI: 10.5455/javar.2024.k838.

Correlation of sperm motility, acrosome integrity, protamine deficiency, and DNA fragmentation in proven and unproven Friesian Holstein bulls.

Handarini R, Baharun A, Rahmi A, Sudrajat D, Anggraeni A, Nurcholis N J Adv Vet Anim Res. 2024; 11(3):796-802.

PMID: 39605780 PMC: 11590584. DOI: 10.5455/javar.2024.k831.

Sperm HSP70: may not be an age-dependent gene but is associated with field fertility in Bali bulls ().

Fatmila D, Pardede B, Maulana T, Said S, Yudi Y, Purwantara B Anim Reprod. 2024; 21(2):e20230048.

PMID: 38756622 PMC: 11095850. DOI: 10.1590/1984-3143-AR2023-0048.

Bovine sperm HSP-70 molecules: a potential cryo-tolerance marker associated with semen quality and fertility rate.

Pardede B, Kusumawati A, Pangestu M, Purwantara B Front Vet Sci. 2023; 10:1167594.

PMID: 37621869 PMC: 10445158. DOI: 10.3389/fvets.2023.1167594.

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