Myoinositol Supplementation of Freezing Medium Improves the Quality-Related Parameters of Dog Sperm

Overview

Journal Animals (Basel)

Date 2019 Dec 1

PMID 31783679

Citations 10

Authors

Ahmad Yar Qamar

Xun Fang

Min Jung Kim

Jongki Cho

Affiliations

Soon will be listed here.

Abstract

Oxidative stress during freeze-thaw procedures results in reduced semen fertility. A decrease in free radical levels can improve the post-thaw sperm quality. We examined the effects of myoinositol supplementation in freezing medium on the structure and function of cryopreserved dog sperm. Pooled ejaculates were diluted with buffer without or with myoinositol (1 or 2 mg/mL). Analysis of fresh semen revealed that the optimal concentration of myoinositol was 1 mg/mL, and this concentration was used in further experiments. Post-thaw semen quality in the myoinositol-supplemented group was superior ( 0.05) compared with that in the control group in terms of motility (57.9 ± 0.4% vs. 47.8 ± 0.2%), sperm viability (57.5 ± 0.5% vs. 44.6 ± 0.6%), intact plasma membrane (56.6 ± 0.4% vs. 46.2 ± 0.6%), and acrosome membrane (59.3 ± 0.5% vs. 51.8 ± 0.5%). In addition, sperm in the myoinositol-supplemented group showed a significantly lower expression of pro-apoptotic () and mitochondrial reactive oxygen species (ROS) modulator () genes but higher expression of anti-apoptotic (), and protamine-related ( and ) genes compared with that in the control group. Therefore, myoinositol supplementation before freezing can protect against oxidative stress and improve post-thaw dog sperm quality.

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