A Lipid-Coated Nanoconstruct Composed of Gold Nanoparticles Noncovalently Coated with Small Interfering RNA: Preparation, Purification and Characterization

Overview

Journal Nanomaterials (Basel)

Date 2021 Nov 27

PMID 34835540

Citations 6

Authors

Anna V Epanchintseva

Julia E Poletaeva

Ilya S Dovydenko

Boris P Chelobanov

Dmitrii V Pyshnyi

Elena I Ryabchikova

Inna A Pyshnaya

Affiliations

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Abstract

There is an urgent need to develop systems for nucleic acid delivery, especially for the creation of effective therapeutics against various diseases. We have previously shown the feasibility of efficient delivery of small interfering RNA by means of gold nanoparticle-based multilayer nanoconstructs (MLNCs) for suppressing reporter protein synthesis. The present work is aimed at improving the quality of preparations of desired MLNCs, and for this purpose, optimal conditions for their multistep fabrication were found. All steps of this process and MLNC purification were verified using dynamic light scattering, transmission electron microscopy, and UV-Vis spectroscopy. Factors influencing the efficiency of nanocomposite assembly, colloidal stability, and purification quality were identified. These data made it possible to optimize the fabrication of target MLNCs bearing small interfering RNA and to substantially improve end product quality via an increase in its homogeneity and a decrease in the amount of incomplete nanoconstructs. We believe that the proposed approaches and methods will be useful for researchers working with lipid nanoconstructs.

Citing Articles

The Photomodification Method Allows for Determining the Composition of the Full and Soft Protein Corona on the Lipid Surface of Composite Nanoparticles.

Epanchintseva A, Baranova S, Poletaeva J, Tupitsyna A, Ryabchikova E, Dovydenko I Nanomaterials (Basel). 2024; 14(23).

PMID: 39683364 PMC: 11643909. DOI: 10.3390/nano14231976.

Study of Hard Protein Corona on Lipid Surface of Composite Nanoconstruction.

Epanchintseva A, Baranova S, Poletaeva J, Bakhno I, Ryabchikova E, Dovydenko I Nanomaterials (Basel). 2024; 14(21).

PMID: 39513847 PMC: 11547845. DOI: 10.3390/nano14211767.

Fixation and Visualization of Full Protein Corona on Lipid Surface of Composite Nanoconstruction.

Epanchintseva A, Poletaeva J, Bakhno I, Belov V, Grigoreva A, Baranova S Nanomaterials (Basel). 2023; 13(24).

PMID: 38132992 PMC: 10745710. DOI: 10.3390/nano13243094.

Chemical Modifications Influence the Number of siRNA Molecules Adsorbed on Gold Nanoparticles and the Efficiency of Downregulation of a Target Protein.

Epanchintseva A, Poletaeva J, Dome A, Dovydenko I, Pyshnaya I, Ryabchikova E Nanomaterials (Basel). 2022; 12(24).

PMID: 36558301 PMC: 9781748. DOI: 10.3390/nano12244450.

Formation of Aggregate-Free Gold Nanoparticles in the Cyclodextrin-Tetrachloroaurate System Follows Finke-Watzky Kinetics.

Pestovsky Y, Srichana T Nanomaterials (Basel). 2022; 12(4).

PMID: 35214912 PMC: 8875903. DOI: 10.3390/nano12040583.

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