Identification, Characterization, and Antioxidant Potential of Subsp. Strains Isolated From Feces of Healthy Infants

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2021 Nov 19

PMID 34795651

Citations 11

Authors

Li Zhao

Song Wang

Jiahuan Dong

Jialu Shi

Jiaqi Guan

Deyu Liu

Fei Liu

Bailiang Li

Guicheng Huo

Affiliations

Soon will be listed here.

Abstract

Increasing evidence has indicated that oxidative stress is associated with the health of infants. , especially subsp. strains, are abundant in the gut microbiota of infants, which may have the potential to ameliorate oxidative damage. Thus, this study aimed to isolate and screen subsp. strains with probiotic characters and antioxidant properties as infants' dietary supplements. In this study, 24 subsp. strains were isolated from 15 healthy infants identified 16S rRNA and heat shock protein 60 (60) sequences. subsp. B13, F2, K4, K5, K10, K13, and K15 strains were selected based on high values obtained from autoaggregation, hydrophobicity, and adhesion assays to HT-29 cells. Among these seven strains, subsp. F2, K5, K10, and K15 were selected according to the high tolerance of gastrointestinal tract conditions compared to subsp. BB-12. Among these four strains, subsp. K5 was susceptible to common antibiotics and showed the highest intestinal epithelial cell proliferation of CCD 841 CoN. Additionally, subsp. K5 showed a strong antioxidant capacity, and its supernatant exhibited better activity of reducing power, hydroxyl radical scavenging, and DPPH radical scavenging than that of the intact cells with cell-free extracts. The findings indicated that subsp. K5 could be used as a probiotic candidate in infant nutrition.

Citing Articles

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