Spin Labeling of Surface Cysteines Using a Bromoacrylaldehyde Spin Label

Overview

Journal Appl Magn Reson

Date 2021 Nov 15

PMID 34776648

Citations 1

Authors

Graham Heaven

Michael A Hollas

Lydia Tabernero

Alistair J Fielding

Affiliations

Soon will be listed here.

Abstract

Supplementary Information: The online version contains supplementary material available at 10.1007/s00723-021-01350-1.

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References

Hintz H, Vanas A, Klose D, Jeschke G, Godt A . Trityl Radicals with a Combination of the Orthogonal Functional Groups Ethyne and Carboxyl: Synthesis without a Statistical Step and EPR Characterization. J Org Chem. 2019; 84(6):3304-3320. DOI: 10.1021/acs.joc.8b03234. View

Sette P, Mu R, Dussupt V, Jiang J, Snyder G, Smith P . The Phe105 loop of Alix Bro1 domain plays a key role in HIV-1 release. Structure. 2011; 19(10):1485-95. PMC: 3195861. DOI: 10.1016/j.str.2011.07.016. View

Yang Y, Yang F, Gong Y, Bahrenberg T, Feintuch A, Su X . High Sensitivity In-Cell EPR Distance Measurements on Proteins using an Optimized Gd(III) Spin Label. J Phys Chem Lett. 2018; 9(20):6119-6123. DOI: 10.1021/acs.jpclett.8b02663. View

Yin D, Hammler D, Peter M, Marx A, Schmitz A, Hagelueken G . Inhibitor-Directed Spin Labelling-A High Precision and Minimally Invasive Technique to Study the Conformation of Proteins in Solution. Chemistry. 2018; 24(26):6665-6671. DOI: 10.1002/chem.201706047. View

Ackermann K, Pliotas C, Valera S, Naismith J, Bode B . Sparse Labeling PELDOR Spectroscopy on Multimeric Mechanosensitive Membrane Channels. Biophys J. 2017; 113(9):1968-1978. PMC: 5685675. DOI: 10.1016/j.bpj.2017.09.005. View

Tzakos A, Grace C, Lukavsky P, Riek R . NMR techniques for very large proteins and rnas in solution. Annu Rev Biophys Biomol Struct. 2006; 35:319-42. DOI: 10.1146/annurev.biophys.35.040405.102034. View

Stefani F, Zhang L, Taylor S, Donovan J, Rollinson S, Doyotte A . UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting. Curr Biol. 2011; 21(14):1245-50. DOI: 10.1016/j.cub.2011.06.028. View

Ali N, Zhang L, Taylor S, Mironov A, Urbe S, Woodman P . Recruitment of UBPY and ESCRT exchange drive HD-PTP-dependent sorting of EGFR to the MVB. Curr Biol. 2013; 23(6):453-61. DOI: 10.1016/j.cub.2013.02.033. View

Bonucci A, Ouari O, Guigliarelli B, Belle V, Mileo E . In-Cell EPR: Progress towards Structural Studies Inside Cells. Chembiochem. 2019; 21(4):451-460. DOI: 10.1002/cbic.201900291. View

10.

Karthikeyan G, Bonucci A, Casano G, Gerbaud G, Abel S, Thome V . A Bioresistant Nitroxide Spin Label for In-Cell EPR Spectroscopy: In Vitro and In Oocytes Protein Structural Dynamics Studies. Angew Chem Int Ed Engl. 2017; 57(5):1366-1370. DOI: 10.1002/anie.201710184. View

11.

Doyotte A, Mironov A, McKenzie E, Woodman P . The Bro1-related protein HD-PTP/PTPN23 is required for endosomal cargo sorting and multivesicular body morphogenesis. Proc Natl Acad Sci U S A. 2008; 105(17):6308-13. PMC: 2359801. DOI: 10.1073/pnas.0707601105. View

12.

Giannoulis A, Ward R, Branigan E, Naismith J, Bode B . PELDOR in rotationally symmetric homo-oligomers. Mol Phys. 2014; 111(18-19):2845-2854. PMC: 4056887. DOI: 10.1080/00268976.2013.798697. View

13.

Lorenzi M, Puppo C, Lebrun R, Lignon S, Roubaud V, Martinho M . Tyrosine-targeted spin labeling and EPR spectroscopy: an alternative strategy for studying structural transitions in proteins. Angew Chem Int Ed Engl. 2011; 50(39):9108-11. DOI: 10.1002/anie.201102539. View

14.

Kim C, Axup J, Schultz P . Protein conjugation with genetically encoded unnatural amino acids. Curr Opin Chem Biol. 2013; 17(3):412-9. PMC: 4284959. DOI: 10.1016/j.cbpa.2013.04.017. View

15.

Valera S, Ackermann K, Pliotas C, Huang H, Naismith J, Bode B . Accurate Extraction of Nanometer Distances in Multimers by Pulse EPR. Chemistry. 2016; 22(14):4700-3. PMC: 4848767. DOI: 10.1002/chem.201505143. View

16.

Hubbell W, Lopez C, Altenbach C, Yang Z . Technological advances in site-directed spin labeling of proteins. Curr Opin Struct Biol. 2013; 23(5):725-33. PMC: 3805720. DOI: 10.1016/j.sbi.2013.06.008. View

17.

Ichioka F, Takaya E, Suzuki H, Kajigaya S, Buchman V, Shibata H . HD-PTP and Alix share some membrane-traffic related proteins that interact with their Bro1 domains or proline-rich regions. Arch Biochem Biophys. 2006; 457(2):142-9. DOI: 10.1016/j.abb.2006.11.008. View

18.

Jeschke G, Sajid M, Schulte M, Godt A . Three-spin correlations in double electron-electron resonance. Phys Chem Chem Phys. 2009; 11(31):6580-91. DOI: 10.1039/b905724b. View

19.

Fielding A, Grazia Concilio M, Heaven G, Hollas M . New developments in spin labels for pulsed dipolar EPR. Molecules. 2014; 19(10):16998-7025. PMC: 6271499. DOI: 10.3390/molecules191016998. View

20.

Abdelkader E, Lee M, Feintuch A, Ramirez Cohen M, Swarbrick J, Otting G . A New Gd(3+) Spin Label for Gd(3+)-Gd(3+) Distance Measurements in Proteins Produces Narrow Distance Distributions. J Phys Chem Lett. 2015; 6(24):5016-21. DOI: 10.1021/acs.jpclett.5b02451. View