Protective Effect of Antioxidant Peptides from Grass Carp Scale Gelatin on the HO-mediated Oxidative Injured HepG2 Cells

Overview

Journal Food Chem

Specialties Chemistry
Nutritional Sciences

Date 2021 Nov 15

PMID 34776311

Citations 23

Authors

Yue-Ming Hu

Su-Zhen Lu

Ya-Si Li

Hui Wang

Yan Shi

Lu Zhang

Zong-Cai Tu

Affiliations

Soon will be listed here.

Abstract

The protective effect of antioxidant peptides from grass carp scale gelatin on hydrogen peroxide (HO)-mediated oxidative injured HepG2 cells was investigated, and the protective mechanism as well as peptide structure were studied. Pretreated with grass carp scale gelatin hydrolysates (GSGH) for 24 h significantly increased the survival rates and decreased the apoptosis rates of HO-mediated oxidative injured HepG2 cells. The increase in SOD, CAT and GPX activities, reduce in ROS level and MDA content, and weaken in damage on cell membrane and DNA could be responsible for the protective effect of GSGH on HO-mediated oxidative injured HepG2 cells. Peptide sequences of GSGH were analyzed by LC-ESI-Q-Orbitrap-MS/MS, and results showed that most of them were low molecular weight peptide at 358-986 Da. Synergistic effect existed among antioxidant peptides and contributed to the strong antioxidant activities of GSGH.

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